期刊文献

Triploid production and performance in hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) 收藏

杂种肉酱（肾上腺fuscoguttatus♀×Epinephelus lanceolatus♂）中的三倍体生产和性能

原文求助发布源

作者

Weiwei Zhang[a][1];Shuyuan Fu[b][1];Xin Fan[a][1];Jie Huang[a];Yesong Liang[a];Xin Wen[a]PersonEnvelope;Jian Luo[a]PersonEnvelope

作者单位

[a]aState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, China[b]bHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, China[1]1These authors contributed equally to this work.

关键词

Triploidy;Hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂);Cold shock treatment;Gonad;

关键词译文

三倍体;杂交油斑（Epcinephelus fuscoguttatus♀×Epinephelus lanceolatus♂）；冷休克处理；性腺；

发布日期

30 January 2023

DOI

10.1016/j.aquaculture.2022.738891

来源信息

Aquaculture ISSN：0044-8486, 2023年, 563卷, Part1期

摘要

The hybrid grouper Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ has high economic value due to its rapid growth and delicious flesh. However, this hybrid is reproductively capable, implying a certain risk of genetic contamination to wild populations. Therefore, this study aimed to produce sterile triploid hybrids using cold shock and examine gonads of triploid individuals. The highest triploid production efficiency (80.00 ± 34.64%) occurred under a 4–6 °C cold-shock, initiated at 6 min after fertilization, and maintained for 17.5 min. The efficiency was confirmed by counting the chromosome number and examining DNA content. The chromosome count of induced triploids was 3n = 72, while diploid fish had 2n = 48. Flow cytometry showed that DNA content in the triploid group was 1.5 times the diploid group. The growth performance of the triploid hybrid was equivalent to that of the diploid hybrid. Histological analysis of the gonadal tissues confirmed that all 18-month-old triploids were sterile, unlike the diploids. Gonad transcriptome analysis showed that the abnormal ovarian development of triploid hybrid groupers may due to the disorders of gamete generation during meiosis. In conclusion, this study explored the optimal conditions for triploid induction in hybrid grouper using cold shock and analyzed the potential for reproduction. These results not only represent a theoretical foundation for the large-scale preparation of triploid hybrid grouper, but also provide a reference case for future studies of grouper breeding.

摘要译文

杂种石斑鱼肾上腺富菌素fuscoguttatus♀×Epinephelus lanceolatus♂由于其快速生长和美味的肉而具有很高的经济价值。但是，这种杂种具有生殖能力，这意味着对野生种群的遗传污染有一定的风险。因此，这项研究旨在使用冷冲击产生无菌三倍体杂种并检查三倍体个体的性腺。最高的三倍体生产效率（80.00±34.64％）发生在4-6°C的冷震下，在受精后6分钟开始，并保持17.5分钟。通过计算染色体数并检查DNA含量来确认效率。诱导三倍体的染色体计数为3n = 72，而二倍体鱼的染色体计数为2n =48。流式细胞仪显示三倍体组中的DNA含量是二倍体组的1.5倍。三倍体杂种的生长性能等效于二倍体杂种。性腺组织的组织学分析证实，与二倍体不同，所有18个月大的三倍体都是无菌的。性腺转录组分析表明，三倍体混合动力学家的异常卵巢发育可能是由于减数分裂过程中配子的产生疾病所致。总之，这项研究探索了使用冷休克探索杂交果中三倍体诱导的最佳条件，并分析了繁殖的潜力。这些结果不仅代表了三倍体混合果仁的大规模制备的理论基础，而且还为将来的石斑鱼育种研究提供了参考案例。

Weiwei Zhang[a][1];Shuyuan Fu[b][1];Xin Fan[a][1];Jie Huang[a];Yesong Liang[a];Xin Wen[a]PersonEnvelope;Jian Luo[a]PersonEnvelope. Triploid production and performance in hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)[J]. Aquaculture, 2023,563(Part1)