摘要
Cow faeces have been investigated as alternative inoculum to replace rumen fluid to determine neutral detergent fibre (NDF) digestibility (NDFD). Aims of this study were to estimate: (1) the NDFD (48 h) of feed ingredients using a rumen inoculum in comparison with faecal inocula from cows fed diets with different forage basis; (2) the undigestible NDF (uNDF) at 240 and 360 h with ruminal fluid and faecal inocula from lactating cows fed two different diets. At 48 h incubation, the NDFD was affected both by feed and type of inoculum (p < 0.01) and by their interaction (p = 0.03). Overall, the mean NDFD was higher for rumen inoculum than for faecal inocula (585 vs. 389 g/kg NDF, p < 0.05), and faecal inoculum obtained from cows fed hay-based diets gave lower NDFD than those from cows fed maize silage (367 vs. 440 g/kg, p < 0.05). At long incubation times, the average uNDF was affected by substrate, inoculum and incubation time (p < 0.01), but not by their interactions. For each inoculum, significantly lower values were obtained at 360 than at 240 h. Regressions between uNDF with rumen and with the tested faecal inocula resulted in r2 ≥ 0.98. Despite the differences at 48 h, the uNDF showed that faecal inoculum could replace rumen fluid at longer incubation times.
摘要译文
已经研究了牛粪物作为替代接种以取代瘤胃流体以确定中性洗涤剂纤维（NDF）消化率（NDFD）。本研究的目的是估计：（1）使用瘤胃接种物的饲料成分的NDFD（48小时）与来自牛喂养饲料的粪便中的粪便接种物相比; （2）在240和360小时的明显NDF（UNDF），患有瘤胃液和粪便喂养牛奶的粪便接种物喂养两种不同的饮食。在48小时温育时，NDFD通过饲料和接种型（P <0.01）和它们的相互作用影响（P = 0.03）。总的来说，对于粪便接种子的瘤胃含量，平均NDFD比粪便接种物（585与389g / kg NDF，P <0.05）和粪便喂养的牛奶饲料中获得的粪便含量低于玉米青贮饲喂的牛奶（367 vs. 440 g / kg，p <0.05）。在长时间孵育时间，平均开发署受基质，接种物和孵育时间（P <0.01）的影响，但不是它们的相互作用。对于每个接种，在360℃下获得显着较低的值，而不是240小时。开支烟结与瘤胃之间的回归和测试的粪便接壤导致R2≥0.98。尽管48小时差异，但开发署显示粪便占状因素可以在较长的培养时间内取代瘤胃液。
Maria Chiaravalli; Luca Rapetti; Andrea Rota Graziosi; Gianluca Galassi; Gianni Matteo Crovetto;Stefania Colombini[ [*]. Comparison of Faecal versus Rumen Inocula for the Estimation of NDF Digestibility[J]. Animals, 2019,9(11): 928