期刊文献

Heat stress and sperm production in the domestic cat 收藏

家猫的热应激和精子产生
原文求助 发布源
作者
R. Nuñez Favre[a][b][1];M.F. García[a][b][1];R. Rearte[a][b];M.C. Stornelli[b];Y.A. Corrada[a][c];R.L. de la Sota[a][b];M.A. Stornelli[b]
作者单位
[a]CONICET, Godoy Cruz, 2290, CABA, C1425FQB, ArgentinaCONICET, Godoy Cruz 2290, CABA, C1425FQB, Argentina[b]Instituto de Investigaciones en Reproduccion Animal, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118, La Plata, B1900AVW, Buenos Aires, ArgentinaInstituto de Investigaciones en Reproduccion Animal, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118, La Plata, B1900AVW, Buenos Aires, Argentina[c]Laboratorio de Fisioterapia, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118, La Plata, B1900AVW, Buenos Aires, ArgentinaLaboratorio de Fisioterapia, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118, La Plata, B1900AVW, Buenos Aires, Argentina[1]1Nuñez Favre R and García MF contributed equally to this work.
发布日期
15 July 2022
页码
182-187
DOI
10.1016/j.theriogenology.2022.05.004
来源信息
Theriogenology ISSN：0093-691X, 2022年, 187卷, 182-187页
摘要
This study aimed to assess 1) the effect of high environmental temperatures on sperm production and 2) the effectiveness of a temperature-humidity index (THI) to predict the degree of thermal stress in a cat model. Semen collection was performed by electroejaculation for 18 mo in 20 tomcats maintained under controlled photoperiod. Still, temperature and humidity were not experimentally manipulated to describe the effect of natural climate conditions on seminal samples. Ejaculates (n = 512) were then grouped according to temperature records of the sampling day and compared by temperature and THI index. Significant lower sperm parameters and increase sperm tail abnormalities were observed during warm environments (temperature and THI). Concentration and total sperm count were the most affected parameters. Environmental temperatures of 28.5 °C with 54% relative humidity (THI = 77.07) and 27.9 °C with 66% humidity (THI = 77.84) were upper thresholds of moderate thermal stress. Moreover, days with relative humidity near 90% led to severe thermal stress with temperatures as low as 26.6 °C (THI = 78.88). The current study demonstrates the detrimental effect of high environmental temperatures on sperm quality in the domestic cat. This effect is observed at lower temperatures when high relative humidity is present. In this sense, the THI was a reliable predictor of the magnitude of thermal stress experienced by cats. Thus, cats from reproductive programs should be maintained under controlled photoperiod cycles with temperatures around 20 °C and humidity around 70% to avoid semen detrimental effects.
摘要译文
这项研究旨在评估1）高环境温度对精子产生的影响； 2）温度 - 湿度指数（THI）预测CAT模型中热应力程度的有效性。通过在受控光周期内维持的20个蛋白猫中的ElectroJaculation进行精液收集。尽管如此，温度和湿度仍未经过实验操纵来描述自然气候条件对开创性样品的影响。然后根据采样日的温度记录对射精（n = 512）进行分组，并根据温度和THI指数进行比较。在温暖的环境（温度和Thi）中观察到明显的较低的精子参数和增加的精子尾异常。浓度和总精子计数是受影响最大的参数。 28.5°C的环境温度为54％的相对湿度（THI = 77.07）和27.9°C，湿度为66％（THI = 77.84）是中等热应力的上阈值。此外，相对湿度接近90％的天数导致严重的热应力，温度低至26.6°C（Thi = 78.88）。当前的研究表明，高环境温度对家猫的精子质量的有害影响。当存在高相对湿度时，在较低温度下观察到这种效果。从这个意义上讲，THI是猫经历的热应激幅度的可靠预测指标。因此，生殖程序的猫应保持在受控光周期的温度下，温度约为20°C，湿度约为70％，以避免精液效果。
R. Nuñez Favre[a][b][1];M.F. García[a][b][1];R. Rearte[a][b];M.C. Stornelli[b];Y.A. Corrada[a][c];R.L. de la Sota[a][b];M.A. Stornelli[b]. Heat stress and sperm production in the domestic cat[J]. Theriogenology, 2022,187: 182-187