摘要
Over the years, rumen fluid transplantation (RT) has been successfully applied to treat acute rumen acidosis in ruminants, but how it functions in the ruminal microbial homeostasis and host function remains largely unknown. Here, we investigated the dynamic changes of rumen fermentation and bacterial communities following RT and its beneficial effects on rumen epithelial morphology and function in a sheep model of rumen acidosis. The results showed that RT resulted in dynamic changes in rumen fermentation and increased the concentrations of total volatile fatty acid, acetate, propionate, and butyrate, but it decreased the levels of lactate and LPS in the rumen. Illumina MiSeq Sequencing data showed that RT facilitated rapid rebuilt of ruminal bacterial homeostasis (8 d in control vs. 2 d in RT) from a markedly dysbiotic acidosis state to a healthy level (similar with those of donors). At the genus level, RT increased the relative abundance of unclassified Bacteroidales, unclassified Prevotellaceae, unclassified Ruminococcaceae, and Acetitomaculum. Additionally, RT also accelerated recovery of the predicted metagenomic function of ruminal bacteria. Rumen papillae morphology results showed that RT alleviated the damage of rumen epithelia induced by acute rumen acidosis and increased the length of rumen papillae. Furthermore, real-time PCR results showed that RT modulated mRNA expression of genes related to cytokines and tight junctions in the rumen epithelia. In summary, these results reveal that RT accelerates recovery of rumen fermentation and bacterial homeostasis and modulates rumen epithelial morphology and function for sheep suffering from rumen acidosis.—Liu, J., Li, H., Zhu, W., Mao, S. Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants. FASEB J. 33, 8453–8467 (2019). www.fasebj.org
摘要译文
多年来，瘤胃流体移植（RT）已成功地应用于治疗反刍动物中的急性瘤胃中毒，但它在瘤胃微生物稳态和宿主功能中如何运作仍然是未知的。在这里，我们研究了RT和其在瘤胃酸中毒中羊模型中瘤胃上皮形态和功能作用后瘤胃发酵和细菌群落的动态变化。结果表明，RT导致瘤胃发酵的动态变化，增加了总挥发性脂肪酸，乙酸盐，丙酸盐和丁酸盐的浓度，但它降低了瘤胃中乳酸水平和LPS。 Illumina MiSeq测序数据显示，从显着的嗜热酸中毒状态（与供体类似），RT促进了瘤胃细菌性稳态（8d在RT中的控制与2d）的快速重新重建。在Genus水平，RT增加了未分类的膀曲的相对丰度，未分类的PRECOTELLACEAE，未分类的喇菇和醋酸粒子。另外，RT也加速了瘤胃细菌预测的偏心函数的回收。 Rumen乳头形态结果表明，RT缓解急性瘤胃酸中毒诱导的瘤胃上皮损伤，增加了瘤胃乳头的长度。此外，实时PCR结果表明，RT调制MRNA表达与瘤胃上皮细胞因子和紧密连接有关的基因。总之，这些结果表明，RT加速瘤胃发酵和细菌性稳态的恢复，并调节瘤胃上皮形态和羊肉患有瘤胃中毒的功能.-刘，J.，Li，H.，朱，W.，Mao，S。瘤胃中瘤瘤中瘤胃移植后瘤胃发酵和细菌群落的动态变化：反刍动物中瘤胃健康的影响。 FASEB J. 33,8453-8467（2019）。 www.fasebj.org.
Junhua Liu[1];Hongwei Li[1];Weiyun Zhu[1];Shengyong Mao[1]. Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants[J]. FASEB Journal, 2019,33(7): 8453-8467