摘要
Ex situ preservation is an important method in the preservation of chickens, and cryopreservation of semen is the only method for gamete preservation at present. During the last two decades, many studies have been performed to develop standard chicken semen cryopreservation technology and achieve great progress. Many attempts and methods were investigated to adapt subspecies or different breeds. In this paper, we firstly reviewed the main factors affecting cryopreservation of chicken sperm, including the unique structure and characteristics of the spermatozoa. Secondly, the studies on key points of the chicken sperm cryopreservation technology, including semen dilution, cryoprotectants, equilibration time, packaging types, and freezing and thawing rates were summarized to generate the optimal parameters. Then, the mechanism underlying freezing damage and freezability revealed by recent omics methods relevant to the efficiency of cryopreservation were discussed. This review will provide relevant reference for the future investigation of poultry semen cryopreservation technology.
摘要译文
事前保存是保存鸡的重要方法，精液的冷冻保存是目前唯一的配子保存方法。在过去的二十年中，已经进行了许多研究，以开发标准的鸡肉精液冷冻保存技术并取得了长足的进步。研究了许多尝试和方法以适应亚种或不同品种。在本文中，我们首先回顾了影响鸡精子冷冻保存的主要因素，包括精子的独特结构和特征。其次，对鸡精子冷冻保存技术的关键点的研究，包括精液稀释，冷冻保护剂，平衡时间，包装类型以及冻结和解冻速率，以产生最佳参数。然后，讨论了与冷冻保存效率相关的最近的OMICS方法所揭示的冻结损伤和冻结性的机制。这篇综述将为家禽精液冷冻保存技术的未来研究提供相关参考。
Yunhe Zong;Yunlei Li;Yanyan Sun;Gamal M. K. Mehaisen;Tianxiao Ma;Jilan Chen. Chicken Sperm Cryopreservation: Review of Techniques, Freezing Damage, and Freezability Mechanisms[J]. Agriculture, 2023,13(2): 445