摘要
The gut microbiota is closely linked to host nutrition, immunity, and health. Here, metagenomic analysis was conducted to elucidate the taxonomic and functional diversity of gut communities from hawksbills and green sea turtles. In terms of diversity and abundance, the gut microbiota of herbivorous green sea turtles showed a higher bacterial diversity and richness than that of hawksbills. Firmicutes dominated in all groups; however, the phylum Proteobacteria showed a higher relative abundance in hawksbills. Several metabolic pathways displayed broad prevalence and high relative abundances in the two sea turtle populations. Antibiotic resistance genes (ARGs) responsible for resistance to glycopeptide and tetracycline were the most abundant in all samples. In ARGs, the subtype macB was the most abundant in the two different sea turtle populations; however, evgS, bcrA, and efrA were more abundant in the green sea turtles, while in the hawksbills, tetT and tetB(P) were more abundant. Among mobile genetic elements (MGEs), the abundance of 16 MGE types showed a significant difference between the two sea turtle populations. MGE type transposase and plasmid were the most abundant in the two sea turtle populations. Additionally, gene functions were enriched in carbohydrate esterases, glycoside hydrolases, and polysaccharide lyases in the green sea turtles, whereas genes related to glycosyltransferases and auxiliary activities were highly abundant in hawksbills. These metagenomic profiles provide further insights into the microbial diversities of the two types of sea turtles and provide valuable information for future conservation efforts.
摘要译文
肠道菌群与宿主的营养，免疫力和健康密切相关。在这里，进行了宏基因组分析，以阐明鹰派和绿海龟的肠道社区的分类和功能多样性。在多样性和丰度方面，草食性绿海龟的肠道菌群比鹰鸟的细菌多样性和丰富性更高。企业在所有群体中占主导地位；然而，门杆菌在鹰虫中显示出较高的相对丰度。在两只海龟种群中，几种代谢途径表现出广泛的流行和较高的相对丰度。在所有样品中，负责对糖肽和四环素抗性的抗生素抗性基因（ARGS）是最丰富的。在Args中，MACB亚型是两种不同的海龟种群中最丰富的。但是，绿海龟中的EVG，BCRA和EFRA更丰富，而在鹰派，Tett和Tetb（P）中更丰富。在移动遗传因素（MGE）中，两种海龟种群之间的16种MGE类型的丰度显示出显着差异。 MGE型转座酶和质粒在两只海龟种群中最丰富。此外，在绿海龟中，基因功能富含碳水化合物酯酯酶，糖苷水解酶和多糖裂解酶，而与糖基转移酶和辅助活性相关的基因在鹰派中高度丰富。这些宏基因组概况提供了对两种海龟的微生物多样性的进一步见解，并为未来的保护工作提供了宝贵的信息。
Chen; Yuan[1];Xia; Zhongrong[2];Li; Hongwei[1]. Metagenomic comparison of gut communities between hawksbills (Eretmochelys imbricata) and green sea turtles (Chelonia mydas)[J]. Archives of Microbiology, 2022,204(8): 1-11