摘要
BackgroundThis study examined the effects of a solid-state fermented feed additive (FFA) on the small intestine histology/morphology, immunity and microbiota of broilers. Two hundred eighty-eight day-old Arbor Acre chicks, were randomly assigned to one of four groups (each group has 6 replicates, with each replicate containing 12 chickens). The negative control (NC; basal diet), the positive control (PC; basal diet +antibiotic 15 ppm), the fermented feed additive low dose (FFL; basal diet + 0.3 kg/t FFA), and the fermented feed additive high dose (FFH; 3 kg/t FFA) with Lactobacillus casei (L.casei).ResultsThe study found that the FFH and FFL groups gained more weight (1-21d) and the FFL and PC diets had better feed conversion ratio (P < 0.05) than the NC from 0-42d. The FFH group had higher villus height (P < 0.05) in the duodenum than the PC and villus height to crypt depth ratio VH/CD compared to PC and FFL groups. The FFL chickens had greater (P < 0.05) jejunal and ileal villus height than PC and NC groups respectively. The FFL group had a higher ileal VH/CD ratio (P < 0.05). Jejunum VH/CD was higher in FFL and FFH (P < 0.05) than PC (P < 0.05). FFH had a smaller thymus than NC (P < 0.05). FFA diets also increased IL-10 expression (P < 0.05). While IL-1 and TLR4 mRNA expression decreased (P < 0.05) compared to NC. The microbiota analysis showed that the microorganisms that have pathogenic properties such as phylum Delsulfobacterota and class Desulfovibriona and Negativicutes was also significantly reduced in the group treated with FFH and PC while microorganisms having beneficial properties like Lactobacillaceae family, Lactobacillus aviarus genus and Lactobacillus spp were also tended to increase in the FFH and FFL fermented feed groups compared to the PC and NC groups.ConclusionThese findings suggested that the FFA diet may modulate cecal microbiota by reducing pathogenic microorganisms such as phylum Delsulfobacterota and class Desulfovibriona and Negativicutes improve beneficial microorganisms like Lactobacillaceae family, Lactobacillus aviarus genus and Lactobacillus spp. While FFA diet also affect immunity, and gene expression related to immunity.
摘要译文
背景本研究研究了固态发酵饲料添加剂（FFA）对肉鸡的小肠组织学/形态，免疫和微生物群的影响。将288八十岁的乔木英亩小鸡随机分配到四组之一（每组具有6个重复，每个重复含12只鸡）。阴性对照（NC；基础饮食），阳性对照（PC；基础饮食 +抗生素15 ppm），发酵的饲料添加剂低剂量（FFL；基础饮食 +0.3 kg/t FFA）和发酵添加剂高剂量（FFH; 3 kg/t ffa）与乳杆菌（L.casei）一起进行。归因研究发现，FFH和FFL组的体重增加（1-21D），FFL和PC饮食具有更好的饲料转换率（P <0.0555 ）比0-42d的NC。与PC和FFL组相比，DuodeNum的FFH组的绒毛高度（P <0.05）高于PC和绒毛高度与隐窝深度比VH/CD的高度。 FFL鸡的空肠和卵形绒毛的高度分别高于PC和NC组。 FFL组具有较高的回肠VH/CD比（P <0.05）。 FFL和FFH（p <0.05）的空肠VH/CD高于PC（P <0.05）。 FFH的胸腺小于NC（p <0.05）。 FFA饮食还增加了IL-10表达（p <0.05）。与NC相比，IL-1和TLR4 mRNA表达降低（P <0.05）。 The microbiota analysis showed that the microorganisms that have pathogenic properties such as phylum Delsulfobacterota and class Desulfovibriona and Negativicutes was also significantly reduced in the group treated with FFH and PC while microorganisms having beneficial properties like Lactobacillaceae family, Lactobacillus aviarus genus and Lactobacillus spp were also tended to increase in the FFH and FFL fermented feed groups compared to the PC and NC groups.ConclusionThese findings suggested that the FFA diet may modulate cecal microbiota by reducing pathogenic microorganisms such as phylum Delsulfobacterota and class Desulfovibriona and Negativicutes improve beneficial microorganisms like Lactobacillaceae family, Lactobacillus Aviarus属和乳杆菌属。尽管FFA饮食也会影响免疫力，并且与免疫相关的基因表达。
Peng; Wentong[1];Talpur; Mir Zulqarnain[1];Zeng; Yuxian[1];Xie; Peipei[1];Li; Jincheng[1];Wang; Songbo[1];Wang; Lina[1];Zhu; Xiaotong[1];Gao; Ping[1];Jiang; Qingyan[1];Shu; Gang[1];Zhang; Haijun[2]. Influence of fermented feed additive on gut morphology, immune status, and microbiota in broilers[J]. BMC Veterinary Research, 2022,18(1): 1-16