摘要
BackgroundThe direct use of medical zinc oxide in feed will be abandoned after 2022 in Europe, leaving an urgent need for substitutes to prevent post-weaning disorders.ResultsThis study investigated the effect of using rapeseed-seaweed blend (rapeseed meal added two brown macroalgae species Ascophylum nodosum and Saccharina latissima) fermented by lactobacilli (FRS) as feed ingredients in piglet weaning. From d 28 of life to d 85, the piglets were fed one of three different feeding regimens (n = 230 each) with inclusion of 0%, 2.5% and 5% FRS. In this period, no significant difference of piglet performance was found among the three groups. From a subset of piglets (n = 10 from each treatment), blood samples for hematology, biochemistry and immunoglobulin analysis, colon digesta for microbiome analysis, and jejunum and colon tissues for histopathological analyses were collected. The piglets fed with 2.5% FRS manifested alleviated intraepithelial and stromal lymphocytes infiltration in the gut, enhanced colon mucosa barrier relative to the 0% FRS group. The colon microbiota composition was determined using V3 and V1-V8 region 16S rRNA gene amplicon sequencing by Illumina NextSeq and Oxford Nanopore MinION, respectively. The two amplicon sequencing strategies showed high consistency between the detected bacteria. Both sequencing strategies indicated that inclusion of FRS reshaped the colon microbiome of weaned piglets with increased Shannon diversity. Prevotella stercorea was verified by both methods to be more abundant in the piglets supplied with FRS feed, and its abundance was positively correlated with colonic mucosa thickness but negatively correlated with blood concentrations of leucocytes and IgG.ConclusionsFRS supplementation relieved the gut lymphocyte infiltration of the weaned piglets, improved the colon mucosa barrier with altered microbiota composition. Increasing the dietary inclusion of FRS from 2.5% to 5% did not lead to further improvements.
摘要译文
背景技术在欧洲2022年后，饲料中的医疗氧化锌的直接使用将被遗弃，迫切需要替代物，以防止断奶后疾病。研究研究了使用油菜籽 - 海藻混合物（油菜膳食添加了两种棕色大型物种Ascrophym的疗效。 Nodosum和Saccharina Latissima）由乳杆菌（FRS）发酵作为仔猪断奶的饲料成分。从生命的D 22到D 85，仔猪用含有0％，2.5％和5％FRS融入三种不同饲养方案（n = 230）中的一种。在此期间，三组中发现了仔猪性能的显着差异。从仔猪的子集（来自每次治疗中的N = 10），收集用于血液学，生物化学和免疫球蛋白分析的血液样品，微生物组分析的结肠Vigesta，以及用于组织病理学分析的Jejunum和结肠组织。饲喂2.5％FRS的仔猪表现出缓解的肠道上皮和基质淋巴细胞浸润，相对于0％FRS组增强结肠粘膜屏障。使用illumina nextseq和牛津纳米孔沟的V3和V1-V8区16SRRNA基因扩增子测序测定结肠微生物酵母组合物。两种扩增子测序策略显示出检测到的细菌之间的高一致性。两个排序策略都表明，包含FRS的含有随后仔猪的结肠微生物组成，随着Shannon多样性增加。 PREVOTLA STERCOREA通过两种方法在提供FRS饲料提供的仔猪中验证，其丰度与结肠粘膜厚度呈正相关，但与白细胞和IgG的血液浓度负相关。结合补充缓解了断奶的肠道淋巴细胞浸润仔猪，改善了微生物酵母组合物改变的结肠粘膜屏障。增加FRS的膳食含量从2.5％到5％不会导致进一步改善。
Hui; Yan[1];Tamez-Hidalgo; Paulina[2];Cieplak; Tomasz[1];Satessa; Gizaw Dabessa[3];Kot; Witold[4];Kjærulff; Søren[2];Nielsen; Mette Olaf[5];Nielsen; Dennis Sandris[1];Krych; Lukasz[1]. Supplementation of a lacto-fermented rapeseed-seaweed blend promotes gut microbial- and gut immune-modulation in weaner piglets[J]. Journal of Animal Science and Biotechnology, 2021,12(1): 1-14