摘要
The study investigated the effects of feeding mixtures of alfalfa (AF) and sweet sorghum (SS) at different ratios of silages in terms of the physiological status of blood and rumen, and rumen microbiota in lambs. A total of 30 four-month-old male Karakul lambs with 25.5 ± 1.4 kg mean initial body weight were randomly allocated to five groups, with six lambs in each group. Five experimental diets containing 40% of one of the five AF–SS mixed silages (containing 0%, 20%, 40%, 60%, and 80% AF on a fresh weight basis, respectively) and 60% of other ingredients were formulated. Overall, the results showed that the mixed silage with more AF tended to increase serum antioxidant capacity, dry matter (DM) intake, and rumen fermentation metabolites. The AF–SS mixed silages containing AF at 60% and 80% caused a significant linear increase (p < 0.05) in the activity of total antioxidant capacity. The superoxide dismutase in the Karakul lamb responded with significant linear and quadratic increases (p < 0.01) as the ratio of AF was increased in the AF–SS mixed silages. Feeding diets with AF in silage mixtures at the ratio of 60% significantly increased (p < 0.05) the concentration of ruminal total volatile fatty acids (tVFA), acetate, and ammonia-N. However, no statistical significance (p > 0.05) was found in the alpha diversity of rumen microbes among the tested groups (p > 0.05). Principal coordinates analysis could clearly discriminate the differences between the five groups (p = 0.001). The relative abundance of Firmicutes in the rumen were significantly higher with AF at 40% in the AF–SS silage-based diet than those with AF at 0%, and 20% ratios. The abundance of Ruminococcus_albus had a significant linear increase (p < 0.05), as the ratio of AF in the AF–SS mixed silages was increased. In conclusion, the best beneficial effect on the physiological status of the blood and rumen, DM intake, and rumen microbiota in lambs came from those that consumed the diet containing the AF–SS mixed silage with 60% AF.
摘要译文
该研究调查了苜蓿（AF）和甜高粱（SS）的喂养混合物的影响，以不同的比例在血液和瘤胃的生理状态以及羔羊中的瘤胃微生物群中。总共30个四个月大的男性卡拉库尔羔羊，平均体重为25.5±1.4 kg，随机分配给五组，每组六只羔羊。五种实验饮食中包含五种AF -SS混合青贮饲料中的40％（分别在重量基础上分别包含0％，20％，40％，60％和80％的AF）和其他成分的60％。 。总体而言，结果表明，具有更多AF的混合青贮饲料倾向于增加血清抗氧化能力，干物质（DM）摄入量和瘤胃发酵代谢物。含有60％和80％的AF的AF – SS混合青贮饲料导致总抗氧化能力的活性显着增加（P <0.05）。卡拉库尔羔羊中的超氧化物歧化酶以明显的线性和二次增加的响应（p <0.01），因为AF -SS混合青贮饲料的AF之比增加。以60％的比率以青贮饲料混合物的喂养饮食显着增加（p <0.05）（p <0.05）瘤胃总挥发性脂肪酸（TVFA），乙酸盐和氨-N的浓度。然而，在测试组中瘤胃微生物的α多样性中未发现统计显着性（p> 0.05）（p> 0.05）。主坐标分析可以清楚地区分五组之间的差异（p = 0.001）。瘤胃中的富公司的相对丰度在基于AF – SS的青贮饲料饮食中的AF的相对丰度比为0％和20％比率的AF饮食的AF显着高于40％。丰富的ruminococcus_albus有明显的线性增加（p <0.05），因为AF -SS混合青贮饲料中AF的比率增加。总之，对羔羊的血液和瘤胃，DM摄入量和瘤胃菌群的生理状态的最佳有益作用来自食用含有60％AF的AF -SS混合青贮饲料的饮食的人。
Jiao Wang;Long Cheng;Abdul Shakoor Chaudhry;Hassan Khanaki;Imtiaz H. R. Abbasi;Yi Ma;Farzana Abbasi;Xuefeng Guo;Sujiang Zhang. Silage Mixtures of Alfalfa with Sweet Sorghum Alter Blood and Rumen Physiological Status and Rumen Microbiota of Karakul Lambs[J]. Animals, 2022,12(19): 2591