摘要
Embryo transfer and the vitrification of embryos could be used for the conservation and recovery of endangered donkey breeds. It is important to develop techniques that optimize recovery rates and the cryotolerance of donkey embryos. This study evaluates factors affecting the recovery rate, quality, and diameter of embryos obtained from donor jennies as a starting point for the use of vitrification and embryo transfer in the conservation of the Andalusian donkey. A total of 100 embryos were recovered out of 124 estrous cycles (80.6%). The donor jenny affected the rates of positive flushings (PFR; p = 0.040) and embryo recovery (ERR; p < 0.05) as well as embryo quality (p = 0.004). ERR was also affected by the number of flushings (p < 0.001), donor age (p < 0.05), successive cycle within donor (p < 0.001), and jacks (p < 0.05). Number of flushings (p < 0.001) and jack (p < 0.05) had a significant effect on PFR, whereas the day of flushing influenced the developmental stage (p < 0.001), embryo quality (p < 0.05), and diameter of embryos (p < 0.001). The number of flushings significantly influenced the diameter (p = 0.038) and embryo developmental stage (p = 0.001), whereas the developmental stage was statistically different between herds (p = 0.020). The factors influencing the success of this assisted reproductive technique were donor jenny, donor age, successive cycle within donor, day of flushing, number of flushings, and jack. The identification of these key points is crucial to achieve a higher efficiency of embryo transfer and vitrification processes, before considering their application in the conservation of endangered donkey breeds.
摘要译文
胚胎转移和胚胎的玻璃化可用于保护和康复濒危驴品种。开发优化恢复率和驴胚胎的阴道率的技术非常重要。本研究评估了影响从捐助迟静获得的胚胎的恢复速率，质量和直径作为使用玻璃化和胚胎转移在安达卢西亚驴保护中获得的胚胎的胚胎直径的因素。将总共100个胚胎中回收124个溶解的循环（80.6％）。捐助者詹尼影响了积极冲洗的速率（PFR; p = 0.040）和胚胎恢复（错误; P <0.05）以及胚胎质量（p = 0.004）。错误也受到冲洗数量的影响（P <0.001），供体年龄（P <0.05），供体内连续循环（P <0.001）和千斤顶（P <0.05）。冲洗次数（P <0.001）和千斤顶（P <0.05）对PFR有显着影响，而冲洗日则影响发育阶段（P <0.001），胚胎质量（P <0.05）和胚胎直径（ p <0.001）。冲洗的数量显着影响直径（p = 0.038）和胚胎发育阶段（p = 0.001），而发育阶段在畜群之间有统计学不同（p = 0.020）。影响这项辅助生殖技术成功的因素是供体珍妮，供体年龄，捐赠者内的连续周期，冲洗的一天，冲洗数和千斤顶。在考虑其在濒危驴品种保护期间之前，这些关键点的鉴定对于实现更高的胚胎转移和玻璃化方法的效率至关重要。
J. Dorado[ 1;[*]; M. Bottrel[ 1]; I. Ortiz[ 1]; M. Díaz-Jiménez[ 1]; B. Pereira[ 1]; C. Consuegra[ 1]; J. J. Carrasco[ 2]; V. Gómez-Arrones[ 2]; A. Domingo[ 2];M. Hidalgo[ 1]. Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies[J]. Animals, 2020,10(11): 1967