摘要
The slug Arion vulgaris has attracted major attention as one of the worst invasive herbivore pests in Europe and is renowned for the stiff mucus it secretes for locomotion. In this study we focused on the isolation and characterisation of extracellular vesicles, specifically exosomes and exosome-like vesicles, from Arion secretions. We developed a method for slug mucus collection and subsequent vesicle isolation by ultracentrifugation. The isolated vesicles with an average diameter of ~ 100 nm carry abundant proteins and short RNAs, as well as adhesion molecules similar to mammalian galectins. We demonstrated that the slug extracellular vesicles are internalised by plant cells and human cancer cells in in vitro assays and are loadable by bioactive compounds, which makes them an interesting tool for utilisation in biotechnology.
摘要译文
Slug Arion dulgaris作为欧洲最严重的侵入性草食动物害虫之一引起了人们的关注，并以其僵硬的粘液为运动而闻名。在这项研究中，我们关注细胞外囊泡的隔离和表征，特别是外泌体和外泌体样囊泡，从阿里奥分泌物中。我们开发了一种通过超速离心进行粘液粘液收集和随后的囊泡分离的方法。平均直径约为100 nm的分离囊泡携带丰富的蛋白质和短RNA，以及类似于哺乳动物乳清蛋白的粘附分子。我们证明了sl骨外囊泡在体外测定中被植物细胞和人类癌细胞内化，并且可以通过生物活性化合物载，这使它们成为生物技术中利用的有趣工具。
Liegertová; Michaela[1];Semerádtová; Alena[1];Kocholatá; Michaela[1];Průšová; Michaela[1];Němcová; Lenka[2];Štofik; Marcel[1];Kříženecká; Sylvie[3];Maly; Jan[1];Janoušková; Olga[1]. Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology[J]. Scientific reports, 2022: 1-9