摘要
3D-printing technology allows the automated and reproducible manufacturing of functional structures for tissue engineering with customized geometries and compositions by depositing materials layer-by-layer with high precision. For these purposes, the production of bioactive gel-based 3D-scaffolds made of biocompatible materials with well-defined internal structure comprising a dual (mesoporous and macroporous) and highly interconnected porosity is essential. In this work, aerogel scaffolds for bone regeneration purposes were obtained by an innovative strategy that combines the 3D-printing of alginate-hydroxyapatite (HA) hydrogels and the supercritical CO2 drying of the gels. BET and SEM analyses were performed to assess the textural parameters of the obtained aerogel scaffolds and the dimensional accuracy to the original computer-aided design (CAD) design was also evaluated. The biological characterization of the aerogel scaffolds was also carried out regarding cell viability, adhesion and migration capacity. The obtained alginate-HA aerogel scaffolds were highly porous, biocompatible, with high fidelity to the CAD-pattern and also allowed the attachment and proliferation of mesenchymal stem cells (MSCs). An enhancement of the fibroblast migration toward the damaged area was observed in the presence of the aerogel formulations tested, which is positive in terms of bone regeneration.
摘要译文
3D印刷技术允许通过高精度沉积材料层和成分的定制几何形状和组合物的组织工程自动和可再生制造。出于这些目的，生产由生物相容性材料制成的生物活性凝胶的3D支架，其具有良好定义的内部结构，包括双（中孔和大孔）和高互连的孔隙度是必不可少的。在这项工作中，通过一种创新的策略来获得用于骨再生目的的气凝胶支架，该策略结合了藻酸盐 - 羟基磷灰石（HA）水凝胶的3D印刷和凝胶的超临界CO 2干燥。进行BET和SEM分析以评估所获得的气凝胶支架的纹理参数，并且还评估了原始计算机辅助设计（CAD）设计的尺寸精度。吸气支架的生物学表征还对细胞活力，粘附性和迁移能力进行。获得的藻酸盐-HA气凝胶支架是高度多孔的，生物相容性，高保真对CAD模式，并且还允许间充质干细胞（MSC）的附着和增殖。在测试的气凝胶制剂存在下观察到朝向受损区域的成纤维细胞迁移的增强，这在骨再生方面是正的。
Ana Iglesias-Mejuto;Carlos A. García-González. 3D-printed alginate-hydroxyapatite aerogel scaffolds for bone tissue engineering[J]. Materials Science and Engineering: C, 2021,131