摘要
ObjectiveThe purpose of this study was to explore the association of expression of cystic fibrosis transmembrane conductance regulator (CFTR) in cumulus cells (CCs) from mature oocytes with oocyte quality and embryonic development.MethodsA total of 338 infertile women who underwent ovarian stimulation cycle of oocyte retrieval in Zhejiang University School of Medicine were retrospectively enrolled in this study. The relative mRNA expression levels of CFTR, bone morphogenetic protein 15 (BMP15), and growth differentiation factor 9 (GDF9) in CCs were detected by qPCR technology. ROC curve was applied for the diagnosis of oocyte maturation. The serum levels of anti-Müllerian hormone (AMH), E2, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and androstenedione were measured. Oocyte maturation rate, fertilization rate, cleavage rate, high-quality embryo formation rate, and implantation rate after embryo transfer were also determined.ResultsThe mRNA expression levels of CFTR in CCs were significantly increased in metaphase II (MII) oocytes compared to that in metaphase I (MI) or germinal vesicle (GV) oocytes. The ROC curve analysis illustrated that CFTR mRNA expression could efficiently discriminate MII oocytes from MI or GV oocytes (AUC = 0.954), and revealed that 0.695 RQU is the optimal cut-off value for diagnosis. So the cut-off value of 2−ΔΔCT = 0.70 was used to divide the patients into two groups: low- (n = 114) and high-CFTR group (n = 224). The mRNA expression of CFTR in CCs was positively correlated with the antral follicular count (AFC), number of oocytes retrieved, number of MII oocytes, serum E2 level on hCG day, and BMP15 and GDF9 expression in CCs. Under continuous stimulation with the same dose of recombinant follicle-stimulating hormone (rFSH), the number of follicles, average recovered oocytes, recovered oocytes, MII oocytes, as well as the oocyte recovery rate, fertilization rate, oocyte cleavage rate, high-quality embryo formation rate, and implantation rate were decreased in patients with lower CFTR.ConclusionsThis study suggests that CFTR expression in CCs is associated with the developmental potential of human oocytes.
摘要译文
本研究的客观性目的是探索从成熟卵母细胞中囊肿细胞中囊性纤维化跨膜电导调节剂（CFTR）与卵母细胞质量和胚胎发育中的表达相关性。Methodsa总计338个中性妇女，这些妇女是卵巢刺激周期的338个中性女性这项研究回顾了郑大学医学院的检索。通过QPCR技术检测到CFTR，骨形态发生蛋白15（BMP15）和生长分化因子9（GDF9）的相对mRNA表达水平。ROC曲线用于诊断卵母细胞成熟。测量了抗肿瘤激素（AMH）的血清水平，E 2，卵泡刺激激素（FSH），黄体生成激素（LH）和雄激素。还确定了卵母细胞的成熟率，受精速率，裂解率，高质量的胚胎形成率和胚胎转移后的植入率。在CCS中，CFTR的CFTR的mRNA表达水平显着增加，在中期II（MII）（MII）的卵母细胞中，与Celsaphase相比I（MI）或生发囊泡（GV）卵母细胞。ROC曲线分析表明，CFTR mRNA表达可以有效地将MII卵母细胞与MI或GV卵母细胞（AUC = 0.954）区分开，并揭示了0.695 RQU是诊断的最佳临界值。因此，使用2 -ΔΔct = 0.70的截止值将患者分为两组：低 - （n = 114）和高CFTR组（n = 224）。CFTR在CCS中的mRNA表达与鼻卵泡计数（AFC），卵母细胞的数量，MII卵母细胞数量，HCG日的血清E 2水平以及CCS中的BMP15和GDF9表达呈正相关。在连续刺激下，具有相同剂量的重组卵泡刺激激素（RFSH），卵泡的数量，平均回收的卵母细胞，恢复的卵母细胞，MII卵母细胞，卵母细胞的回收率，卵母细胞裂解速率，卵母细胞裂解速率，卵母细胞恢复速率，卵母细胞恢复速率较低的CFTR concconconions的患者的胚胎形成率和植入率降低，这表明CCS中的CFTR表达与人卵母细胞的发育潜力有关。
Yang; Jing[1];Wang; Ming-Juan[1];Huang; Wen-Jie[1];Zhou; Qian[1];Ying; Xue[1];Tian; Yong-Hong[1];Zhu; Yi-Min[1];Lu; Yong-Chao[1]. High expression of CFTR in cumulus cells from mature oocytes is associated with high-quality of oocyte and subsequent embryonic development[J]. Journal of Assisted Reproduction and Genetics, 2022,39(10): 2239-2247