摘要
BackgroundIn modern animal husbandry, breeders pay increasing attention to improving sow nutrition during pregnancy and lactation to favor the health of neonates. Sow milk is a main food source for piglets during their first three weeks of life, which is not only a rich repository of essential nutrients and a broad range of bioactive compounds, but also an indispensable source of commensal bacteria. Maternal milk microorganisms are important sources of commensal bacteria for the neonatal gut. Bacteria from maternal milk may confer a health benefit on the host.MethodsSow milk bacteria were isolated using culturomics followed by identification using 16S rRNA gene sequencing. To screen isolates for potential probiotic activity, the functional evaluation was conducted to assess their antagonistic activity against pathogens in vitro and evaluate their resistance against oxidative stress in damaged Drosophila induced by paraquat. In a piglet feeding trial, a total of 54 newborn suckling piglets were chosen from nine sows and randomly assigned to three treatments with different concentrations of a candidate strain. Multiple approaches were carried out to verify its antioxidant function including western blotting, enzyme activity analysis, metabolomics and 16S rRNA gene amplicon sequencing.ResultsThe 1240 isolates were screened out from the sow milk microbiota and grouped into 271 bacterial taxa based on a nonredundant set of 16S rRNA gene sequencing. Among 80 Pediococcus isolates, a new Pediococcus pentosaceus strain (SMM914) showed the best performance in inhibition ability against swine pathogens and in a Drosophila model challenged by paraquat. Pretreatment of piglets with SMM914 induced the Nrf2-Keap1 antioxidant signaling pathway and greatly affected the pathways of amino acid metabolism and lipid metabolism in plasma. In the colon, the relative abundance of Lactobacillus was significantly increased in the high dose SMM914 group compared with the control group.ConclusionP. pentosaceus SMM914 is a promising probiotic conferring antioxidant capacity by activating the Nrf2-Keap1 antioxidant signaling pathway in piglets. Our study provided useful resources for better understanding the relationships between the maternal microbiota and offspring. The video is not available.Video Abstract
摘要译文
背景饲养现代动物，育种者越来越关注在怀孕和哺乳期间改善母猪营养，以偏爱新生儿的健康。母牛牛奶是生命的头三周的仔猪的主要食物来源，这不仅是必需营养素和广泛的生物活性化合物的丰富仓库，而且是共同细菌的必不可少来源。母牛奶微生物是新生儿肠道的共生细菌的重要来源。孕妇牛奶的细菌可能赋予宿主的健康益处。使用培养基学分离牛奶细菌，然后使用16S rRNA基因测序进行鉴定。为了筛选潜在益生菌活性的分离株，进行了功能评估，以评估其在体外对病原体的拮抗活性，并评估其对帕拉qaraquat诱导的受损果蝇中氧化应激的抗性。在一项小猪喂养试验中，从9种母猪中选择了54头新生儿哺乳小猪，并随机分配给具有不同浓度的候选菌株的三种治疗方法。采用多种方法来验证其抗氧化剂功能，包括蛋白质印迹，酶活性分析，代谢组学和16S rRNA基因基因扩增子测序。回流从母猪牛奶牛奶中筛选了1240个分离株，并将基于16S的细菌分类筛选为16S的细菌分类。 rRNA基因测序。在80个小体育菌分离株中，新的五角面菌株（SMM914）在针对猪病原体的抑制能力和受Paraquat挑战的果蝇模型中表现出最佳性能。用SMM914进行预处理诱导NRF2-KEAP1抗氧化信号通路，并极大地影响了血浆中氨基酸代谢和脂质代谢的途径。在结肠中，与对照组相比，高剂量SMM914组的乳杆菌的相对丰度显着增加。 Pentosaceus SMM914是通过激活小猪中的NRF2-KEAP1抗氧化信号通路来赋予抗氧化能力的有希望的益生菌。我们的研究提供了有用的资源，以更好地了解母体微生物群和后代之间的关系。视频不可用。
Wang; Leli[1];Liu; Qihang[1];Chen; Yuwei[1];Zheng; Xinlei[1];Wang; Chuni[1];Qi; Yining[1];Dong; Yachao[1];Xiao; Yue[1];Chen; Cang[1];Chen; Taohong[1];Huang; Qiuyun[1];Zhai; Zongzhao[1];Long; Cimin[2];Yang; Huansheng[1];Li; Jianzhong[1];Wang; Lei[1];Zhang; Gaihua[3];Liao; Peng[2];Liu; Yong-Xin[4];Huang; Peng[5];Huang; Jialu[5];Wang; Qiye[1];Chu; Huanhuan[6];Yin; Jia[1];Yin; Yulong[2]. Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets[J]. Microbiome, 2022,10(1): 1-19