摘要
Traditional fermented foods are the ideal source of novel probiotic isolates which are known to have significant therapeutic benefits and play a vital role as bioprotective agents. Bhaati jaanr is an ethnic fermented rice beverage popularly consumed in sub-Himalayan regions. The strain UAMS was isolated from Bhaati jaanr based on high butyrate production and evaluated for the potential probiotic characteristics. MALDI-TOF MS and 16 s rRNA gene sequencing revealed the identity of strains as Pediococcus acidilactici. The isolated strain exhibited high tolerance to gastric and bile stress, autoaggregation, hydrophobicity, and adherence to colon cells. Antibiotic susceptibility testing results showed the resistance of the isolated strain toward tested common antibiotics and the pathogenic determinants were absent in PCR-based detection. Moreover, the organism was able to inhibit the growth of Listeria, Salmonella, Staphylococcus, and Enterococcus species. The isolate was found to be a high butyrate producer along with other short-chain fatty acids and exhibited an anti-proliferative effect against colon cancer cells HT29 and SW480. Therefore, our study represents Pediococcus acidilactici UAMS as a potent putative probiotic with bioprotective abilities.
摘要译文
传统的发酵食品是新型益生菌分离株的理想来源，益生菌分离株具有显着的治疗益处，并作为生物保护剂起着至关重要的作用。Bhaati Jaanr是一种在亚马拉亚山区地区盛产的族裔稻米饮料。基于高丁酸酯的产生，将菌株UAM从Bhaati Jaanr分离，并评估了潜在的益生菌特征。MALDI-TOF MS和16 s rRNA基因测序揭示了菌株作为酸虫菌的身份。分离的菌株对胃和胆汁应激，自身凝聚，疏水性和对结肠细胞的遵守表现出很高的耐受性。抗生素易感性测试结果表明，在基于PCR的检测中，分离的菌株对测试的常见抗生素的抗性和致病性决定因素不存在。此外，该生物体能够抑制李斯特菌，沙门氏菌，葡萄球菌和肠球菌的生长。发现分离株是一种高丁酸酯生产剂，以及其他短链脂肪酸，对结肠癌细胞HT29和SW480产生了抗增殖作用。因此，我们的研究代表了酸性化的酸性化合物作为具有生物保护能力的有效推定益生菌。
Jaiswal; Sandeep[1];Pradhan; Satya Narayan[1];Jain; Diamond[1];Dhassiah Peter; Magesh Peter[2];Antony; Usha[1]. Probiotic and Functional Characterization of Pediococcus acidilactici Isolated from Bhaati jaanr, Traditional Fermented Rice Porridge[J]. Applied Biochemistry and Biotechnology, 2022,194(12): 5734-5747