期刊文献

Alteration of RNA modification signature in human sperm correlates with sperm motility 收藏

人类精子中RNA修饰签名的改变与精子运动相关

原文求助发布源

作者

Huanping Guo [1]; Xipeng Shen [1]; Hua Hu [2]; Peng Zhou [2]; Tong He [1]; Lin Xia [1]; Dongmei Tan [3]; Xi Zhang [1]; Yunfang Zhang [1]

作者单位

[1]Medical Center of Hematology, The Second Affiliated Hospital of Army Medical University, Chongqing, China;[2]Center for Reproductive & Genetic Medical, The Second Affiliated Hospital of Army Medical University, Chongqing, China;[3]Laboratory Animal Center, Chongqing Medical University, Chongqing, China

关键词

RNA modification;sperm RNA;epigenetic information;male infertility;asthenozoospermia;teratozoospermia;sperm motility

关键词译文

RNA修饰；精子RNA；表观遗传信息；雄性不育症；哮喘植物； teratozoospermia;精子运动

发布日期

12 August 2022

DOI

10.1093/molehr/gaac031

来源信息

Molecular Human Reproduction ISSN：e1460-2407, 2022年, 28卷, 9期

摘要

RNA modifications, which are introduced post-transcriptionally, have recently been assigned pivotal roles in the regulation of spermatogenesis and embryonic development. However, the RNA modification landscape in human sperm is poorly characterized, hampering our understanding about the potential role played by RNA modification in sperm. Through our recently developed high-throughput RNA modification detection platform based on liquid chromatography with tandem mass spectroscopy, we are the first to have characterized the RNA modification signature in human sperm. The RNA modification signature was generated on the basis of 49 samples from participants, including 13 healthy controls, 21 patients with asthenozoospermia (AZS) and 15 patients with teratozoospermia (TZS). In total, we identified 13 types of RNA modification marks on the total RNA in sperm, and 16 types of RNA modification marks on sperm RNA fragments of different sizes. The levels of these RNA modifications on the RNA of patients with AZS or TZS were altered, compared to controls, especially on sperm RNA fragments >80 nt. A few types of RNA modifications, such as m¹G, m⁵C, m²G and m¹A, showed clear co-expression patterns as well as high linear correlations with clinical sperm motility. In conclusion, we characterized the RNA modification signature of human sperm and identified its correlation with sperm motility, providing promising candidates for use in clinical sperm quality assessment and new research insights for exploring the underlying pathological mechanisms in human male infertility syndromes.

摘要译文

在转录后引入的RNA修饰最近已在精子发生和胚胎发育的调节中分配了关键作用。然而，人类精子中的RNA修饰景观的特征很差，这阻碍了我们对RNA修饰在精子中所起的潜在作用的理解。通过我们最近开发的高通量RNA修饰检测平台，基于液相色谱和串联质谱，我们是第一个表征人类精子中RNA修饰签名的人。 RNA修饰签名是根据参与者的49个样本（包括13个健康对照组，21例哮喘患者（AZS）和15例Teratozoospermia（TZS）患者产生的。我们总共确定了精子中总RNA上的13种类型的RNA修饰标记，在不同大小的精子RNA片段上的16种RNA修饰标记。与对照组相比，对AZS或TZ患者RNA的这些RNA修饰的水平改变了，尤其是在> 80 nt的精子RNA片段上。几种类型的RNA修饰，例如M ¹ g，m ⁵ c，m ² g and m ¹ > a显示清晰的共表达模式以及与临床精子运动的高线性相关性。总之，我们表征了人类精子的RNA修饰特征，并确定了其与精子运动的相关性，为临床精子质量评估和新的研究见解提供了有希望的候选者，以探索人类男性不育症综合症的潜在病理机制。

Huanping Guo [1]; Xipeng Shen [1]; Hua Hu [2]; Peng Zhou [2]; Tong He [1]; Lin Xia [1]; Dongmei Tan [3]; Xi Zhang [1]; Yunfang Zhang [1]. Alteration of RNA modification signature in human sperm correlates with sperm motility[J]. Molecular Human Reproduction, 2022,28(9)