期刊文献

Identification of a gene induced in conjugation-promoted cells of toxic marine dinoflagellates Alexandrium tamarense and Alexandrium catenella using differential display analysis 收藏

用差异显示分析诱发有毒海洋甲藻亚历山大藻和链状亚历山大藻结合，促进细胞的基因的鉴定

原文求助发布源

作者

Shoko Hosoi-Tanabe[1,*]; Sayaka Tomishima[2]; Satoshi Nagai[3] and Yoshihiko Sako[2];

作者单位

[1] Department of Ecosystem Studies, School of Environmental Science, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan [2] Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan [3] Fisheries Research Agency of Japan, National Research Institute of Fisheries and Environment of Inland Sea, Harmful Algal Bloom Division, Toxic Phytoplankton Section, Ohno, Saeki, Hiroshima 739-0452, Japan

关键词

Toxic dinoflagellate; Alexandrium; Paralytic shellfish poisoning; Encystment; Differential display; Sporulation-specific gene

关键词译文

塔玛;亚历山大;麻痹性贝类中毒;包囊;差异显示;孢子形成特异基因

发布日期

9 JAN 2006

页码

161-168

DOI

10.1016/j.femsle.2005.07.046

来源信息

FEMS Microbiology Letters ISSN：0378-1097, 2005年, 251卷, 1期, 161-168页

摘要

Marine dinoflagellates Alexandrium tamarense and Alexandrium catenella produce toxins that cause paralytic shellfish poisoning (PSP). A detailed mechanism of encystment is necessary for a better understanding of bloom dynamics and the toxic effect of these organisms. In this study, a cDNA that was up-regulated in conjugation-promoted cells at encystment was identified using differential display. It encoded a polypeptide of 195 amino acids with a molecular weight of 20,900 Da. The deduced amino acid sequence of this cDNA showed 62% similarity with the polypeptide encoded by SPS19, a gene that is activated specifically during spore maturation and spore wall formation in Saccharomyces cerevisiae. Therefore, the cDNA obtained was termed an SPS19 homolog in this study. The expression levels of the SPS19 homolog were highest immediately after the promotion of conjugation and decreased sequentially later, a pattern similar to that of SPS19 in the sporulation of S. cerevisiae in terms of the time of induction and the duration of expression. These similarities between the SPS19 homolog and SPS19 suggested that the putative function of the SPS19 homolog might be an involvement in encystment. RT-PCR showed that the expression of the SPS19 homolog was highest in conjugation-promoted cells but low in vegetative cells. The SPS19 homolog was believed to be expressed constantly in order for cells to respond rapidly to environmental changes and ensure encystment. Characterization of the identified gene might help in understanding the mechanism of encystment.

摘要译文

海洋甲藻亚历山大藻和链状亚历山大藻产生毒素引起麻痹性贝毒（PSP）。包囊的详细机制是必要为了更好地理解盛开动力学和这些生物体的毒性作用。在这项研究中，在包囊被上调偶联助细胞的cDNA鉴定利用差异显示。它编码195个氨基酸的多肽与20,900达的分子量。该cDNA推导的氨基酸序列显示62％的相似性与由SPS19编码的多肽，它们是在孢子成熟和孢子壁形成在酿酒酵母特异性激活的基因。因此，所获得的基因被命名在本研究的SPS19同源。所述SPS19同源物的表达水平最高立即促进缀合后，并依次以后类似于SPS19的图案中的S的孢子形成减少，酵母中的感应的时间和表达的持续时间而言。的SPS19同源物和SPS19之间的类似建议，SPS19同源的推定功能可能是在包囊的参与。RT-PCR检测表明，SPS19同源的表达量最高的结合，促进细胞，但在低营养细胞。该SPS19同源被认为是不断表示，为了让细胞迅速应对环境变化，并确保包囊。鉴定的基因的鉴定可能有助于理解包囊的机制。

Shoko Hosoi-Tanabe[1,*]; Sayaka Tomishima[2]; Satoshi Nagai[3] and Yoshihiko Sako[2];. Identification of a gene induced in conjugation-promoted cells of toxic marine dinoflagellates Alexandrium tamarense and Alexandrium catenella using differential display analysis[J]. FEMS Microbiology Letters, 2005,251(1): 161-168