摘要
In ovine species, transcervical artificial insemination (TCAI) is limited by the poor quality of frozen–thawed semen and by the convoluted cervical lumen hampering the passage of inseminating devices. The aim of the study was to test the efficiency of three newly designed catheters with bent tips of 3.5 mm, 5.0 mm or 8.0 mm in terms of reproductive performances (experiment 1) and to compare the results of TCAI with the best performing catheter of experiment 1 to those obtained in ewes submitted to surgical incision of cervical folds (SICF) prior to insemination (experiment 2). The following parameters were assessed: time to pass the cervix; depth of cervical penetration; site of deposition of semen; pregnancy (PR); and lambing rates (LR). The results of experiment 1 indicated that the 5.0 mm tip catheter resulted in deeper and faster TCAI and higher PR and LR compared to 3.5 mm and 8.0 mm tip catheters (p < 0.05). In experiment 2, TCAI with the 5.0 mm catheter did not differ from TCAI after SICF in terms of depth of semen deposition, time to pass the cervix, PR and LR (p < 0.05). In conclusion, the use of a catheter that allowed transcervical uterine deposition of semen without excessive manipulation led to satisfactory pregnancy rates.
摘要译文
在绵羊物种中，经血管术人工授精（TCAI）受到冷冻解冻精液的质量差的限制，并通过妨碍衬里静态装置通过的复杂的宫颈内腔。该研究的目的是在生殖性能（实验1）方面测试三个新设计的导管的效率，弯曲尖端为3.5毫米，5.0毫米或8.0毫米，并将TCAI的结果与实验最佳导管进行比较1到在授精之前提交给颈椎折叠（SICF）的手术切口的母羊中获得的那些（实验2）。评估以下参数：通过子宫颈的时间;宫颈渗透的深度;精液沉积部位;怀孕（公关）;和羔羊率（LR）。实验1的结果表明，5.0mm尖端导管导致TCAI和更高的TCAI和更高的PR和LR，而3.5 mm和8.0 mm尖端导管（P <0.05）。在实验2中，用5.0mm导管的TCAI在SICF在精液沉积的深度方面没有与TCAI不同的情况下，通过子宫颈，PR和LR（P <0.05）。总之，使用导管允许精液的转基源性子宫沉积而不过度操作导致令人满意的妊娠率。
Laura Falchi;Maria Teresa Zedda;Salvatore Pau;Mauro Ledda;Valentino Melosu;Salvatore Pier Giacomo Rassu. The Design of a New Catheter for Transcervical Artificial Insemination in Ewes[J]. Animals, 2021,11(12): 3348