期刊文献

First pregnancies in jennies with vitrified donkey semen using a new warming method 收藏

Jennies的首次怀孕与玻璃化驴精液使用新的变暖方法
原文求助 发布源
作者
M. Diaz-Jimenez[a][b];A. Rota[b];J. Dorado[a];C. Consuegra[a];B. Pereira[a];F. Camillo[b];D. Panzani[b];D. Fanelli[b];M. Tesi[b];D. Monaco[c];M. Hidalgo[a]
作者单位
[a]aVeterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, SpainVeterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain[b]bDepartment of Veterinary Sciences, University of Pisa, 56121 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56121 Pisa, Italy[c]cDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70121 Bari, ItalyDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70121 Bari, Italy
关键词
Fertility;Jack;Sperm;Thawing;Vitrification;
关键词译文
生育力;杰克;精子;解冻;玻璃化;
发布日期
1 January 2021
DOI
10.1016/j.animal.2020.100097
来源信息
animal ISSN：1751-7311, 2021年, 15卷, 1期
摘要
Sperm vitrification has been recently developed, but fertility trials have not been performed yet in equine species. In this study, a new warming technique for vitrified donkey semen was developed and the uterine inflammatory response and fertility were compared to conventional freezing. In Experiment 1, sperm was vitrified in straws and warmed in 3 ml of extender or in a water bath at: 37 °C/30 s; 43 °C/10 s; and 60 °C/5 s. Sperm motility, plasma and acrosome membranes and DNA integrity were compared between treatments. In Experiment 2, jennies were inseminated twice (500 × 106 sperm) in the uterine body either with vitrified or frozen semen (2 cycles/jenny). Pregnancy rates and the uterine inflammatory response (polymorphonuclear neutrophil concentration; PMN) were evaluated after artificial insemination (AI). No differences between warming in extender/water bath were found and 43 °C/10 s was better than lower temperatures in terms of total (53.8 ± 13.2%) and progressive sperm motility (41.4 ± 11.4%). No differences in PMN concentration (× 103 PMN/ml) were found between vitrified (276.8 ± 171.6) or frozen (309.7 ± 250.7) semen after AI. However, PMN decreased faster (P < 0.05) using vitrified semen. Pregnancy rates were greater for vitrified (22%) than frozen semen (10%) but not statistically different. In conclusion, donkey sperm vitrified in straws could be directly warmed in a water bath at 43 °C/10 s, reducing the uterine inflammatory response obtained after AI and promoting positive pregnancy outcomes. These findings confirm the possibility to use vitrified semen as an alternative for AI in jennies.
摘要译文
精子玻璃化已被开发，但尚未在马物种中进行生育试验。在这项研究中，开发了一种用于玻璃化驴精液的新变暖技术，与传统冷冻相比，子宫炎症反应和生育率进行了比较。在实验1中，精子在吸管中玻璃化并在3ml的增量剂或水浴中温热：37℃/ 30s; 43°C / 10s;和60°C / 5s。在治疗中比较了精子活力，血浆和血浆膜和DNA完整性。在实验2中，Jennies在子宫体中巩固了两次（500×10 6个精子，其中包含玻璃体或冷冻精液（2鲸/珍妮）。妊娠率和子宫炎症反应（多核核心粒细胞浓度; PMN）在人工授精后评估（AI）。在增量剂/水浴中升温之间没有差异，43°C / 10s比总计（53.8±13.2％）和进步精子运动（41.4±11.4％）更低的温度。在AI之后，在玻璃化（276.8±171.6）或冷冻（309.7±250.7）精液之间，发现PMN浓度（×10 3 pmn / ml）的差异。然而，使用玻璃化精液，PMN降低了更快的（P <0.05）。葡萄酒（22％）比冷冻精液（10％）更大，但妊娠率更大，但没有统计学不同。总之，在43°C / 10s的水浴中玻璃体中的驴子可以直接在水浴中加热，降低AI后获得的子宫炎症反应并促进阳性妊娠结果。这些调查结果证实了将玻璃化精液作为吉恩尼亚州AI的替代方案。
M. Diaz-Jimenez[a][b];A. Rota[b];J. Dorado[a];C. Consuegra[a];B. Pereira[a];F. Camillo[b];D. Panzani[b];D. Fanelli[b];M. Tesi[b];D. Monaco[c];M. Hidalgo[a]. First pregnancies in jennies with vitrified donkey semen using a new warming method[J]. animal, 2021,15(1)