摘要
Intramuscular fat (IMF)—an important factor affecting meat quality—can be appropriately increased by genetic selection. Chicken lines divergently selected for IMF content were used in this study to investigate the mechanisms behind differential IMF deposition. Sixty 15th generation chickens were genotyped using the IASCHICK 55K single nucleotide polymorphism (SNP) chip. After quality control, 59 chickens and 36,893 SNPs were available for subsequent analysis. Population structure assessment indicated that the lines were genetically differentiated. Based on the top 1% paired fixation index values, three pathways were significantly (p < 0.05) enriched, and nine genes were considered candidate genes for differential IMF deposition. Differences between the lines in the expressions of representative genes involved in the above pathways were detected in 16th generation chickens. This study suggests that genetic selection for increased IMF in the pectoralis major muscle may enhance fatty acid synthesis, transport, and esterification, and reduce triglyceride hydrolysis. The peroxisome proliferator-activated receptor (PPAR) signaling pathway, glycerolipid metabolism, and fatty acid degradation pathway may have contributed to the differences in IMF deposition between the lines. These results contribute to the understanding of the genetic mechanisms behind IMF deposition, and the improvement of chicken meat quality.
摘要译文
肌内脂肪（IMF） - 影响肉质的重要因素 - 可以通过遗传选择适当地增加。在该研究中使用了针对IMF含量的鸡排水含有脱脂含量，以研究差异IMF沉积后面的机制。使用IASchick 55K单核苷酸多态性（SNP）芯片进行六十六代鸡。在质量控制后，59只鸡和36,893个SNP可用于随后的分析。人口结构评估表明该系列遗传分化。基于前1％成对的固定指标值，三种途径显着（P <0.05）富集，九个基因被认为是差异IMF沉积的候选基因。在第16代鸡中检测到上述途径中涉及的代表性基因表达之间的差异。本研究表明，胸胸主要肌肉中IMF增加的遗传选择可以增强脂肪酸合成，运输和酯化，并降低甘油三酯水解。过氧化物体增殖物激活的受体（PPAR）信号传导途径，甘油脂代谢和脂肪酸降解途径可能导致线路之间的IMF沉积的差异。这些结果有助于了解IMF沉积背后的遗传机制，以及鸡肉质量的提高。
Lu Liu[ 1;2;†]; Huanxian Cui[ 1;2;†]; Siyuan Xing[ 1;2;3]; Guiping Zhao[ 1;2;[*];Jie Wen[ 1;2;[*]. Effect of Divergent Selection for Intramuscular Fat Content on Muscle Lipid Metabolism in Chickens[J]. Animals, 2020,10(1): 4