摘要
Recent studies have shown promise for the use of probiotics in modulating behaviour through the microbiota–gut–brain axis. In the present study, we assessed the impact of two probiotic strains in mitigating autism-related symptomology in the BTBR T+ Itpr3tf/J mouse model of autism spectrum disorder (ASD). Male juvenile BTBR mice were randomized into: (1) control, (2) Lr probiotic (1 × 109 CFU/mL Lacticaseibacillus rhamnosus HA-114), and (3) Ls probiotic groups (1 × 109 CFU/mL Ligilactobacillus salivarius HA-118) (n = 18–21/group), receiving treatments in drinking water for 4 weeks. Gut microbiota profiling by 16S rRNA showed Lr, but not Ls supplementation, to increase microbial richness and phylogenetic diversity, with a rise in potential anti-inflammatory and butyrate-producing taxa. Assessing serum and brain metabolites, Lr and Ls supplementation produced distinct metabolic profiles, with Lr treatment elevating concentrations of potentially beneficial neuroactive compounds, such as 5-aminovaleric acid and choline. As mitochondrial dysfunction is often observed in ASD, we assessed mitochondrial oxygen consumption rates in the prefrontal cortex and hippocampus. No differences were observed for either treatment. Both Lr and Ls treatment reduced behavioural deficits in social novelty preference. However, no changes in hyperactivity, repetitive behaviour, and sociability were observed. Results show Lr to impart positive changes along the microbiota–gut–brain axis, exhibiting beneficial effects on selected behaviour, gut microbial diversity, and metabolism in BTBR mice.
摘要译文
最近的研究表明，有望通过微生物群 - 肠脑轴调节行为。在本研究中，我们评估了两种益生菌菌株在减轻自闭症相关症状的影响中，自闭症自闭症谱系障碍（ASD）中的BTBR T+ ITPR3TF/J小鼠模型。 Male juvenile BTBR mice were randomized into: (1) control, (2) Lr probiotic (1 × 109 CFU/mL Lacticaseibacillus rhamnosus HA-114), and (3) Ls probiotic groups (1 × 109 CFU/mL Ligilactobacillus salivarius HA- 118）（n = 18–21/组），在饮用水中接受4周的治疗。 16S rRNA的肠道菌群分析表现出LR，但没有补充LS，以增加微生物的丰富性和系统发育多样性，并增加了潜在的抗炎和产生丁酸酯的分类群。评估血清和脑代谢产物，LR和LS补充产生了不同的代谢谱，LR处理升高了潜在的有益神经活性化合物的浓度，例如5-氨基甲甲甲基酸和胆碱。由于在ASD中经常观察到线粒体功能障碍，因此我们评估了前额叶皮层和海马的线粒体氧的消耗率。任何一种治疗都没有观察到差异。 LR和LS治疗都减少了社会新颖性偏好的行为缺陷。但是，没有观察到多动，重复行为和社交性的变化。结果表明，LR沿微生物群 - 脑桥轴散发正变化，对BTBR小鼠的选定行为，肠道微生物多样性和代谢表现出有益的影响。
Angela Pochakom;Chunlong Mu;Jong M. Rho;Thomas A. Tompkins;Shyamchand Mayengbam;Jane Shearer. Selective Probiotic Treatment Positively Modulates the Microbiota–Gut–Brain Axis in the BTBR Mouse Model of Autism[J]. Brain Sciences, 2022,12(6): 781