期刊文献

Diacerein ameliorates letrozole-induced polycystic ovarian syndrome in rats 收藏

透明蛋白可以改善letrozole诱导的大鼠多囊卵巢综合征
摘要
Polycystic ovary syndrome (PCOS) is the most common gynaecological endocrine disease that causes anovulatory infertility. The current study aimed to explore the possible role of diacerein (DIA), an IL-1β inhibitor, in treating letrozole-induced PCOS in rats that exhibit the metabolic and endocrinal criteria of PCOS patients. PCOS was induced in female Wistar rats by the oral administration of letrozole (1 mg/kg, per orally, p.o.) for 21 days. Rats were then treated with DIA (25 mg/kg/day, p.o.), DIA (50 mg/kg/day, p.o.), or metformin (2 mg/100 g/day, p.o.) for 14 days after the PCOS induction. PCOS resulted in a significantly higher body weight, ovarian weight, ovarian size, and cysts, as well as an elevation in serum testosterone, LH, insulin, glycemia, and lipid profile levels. All of these effects were significantly reduced by the DIA administration. Additionally, DIA remarkably inhibited the letrozole-induced oxidative stress in the ovaries, muscles, and liver by reducing the upraised levels of malondialdehyde and total nitrite and increasing the suppressed levels of superoxide dismutase and catalase. DIA enhanced the protective proteins Keap-1, Nrf2, and OH-1 levels. Finally, DIA inhibited the elevated mRNA levels of NLRP3 and caspase-1, the up-regulated inflammatory cytokines IL-6, TNF-α, and the IL-1β/NFκB signaling pathway. Our results proved that DIA ameliorates letrozole-induced PCOS through its antioxidant and anti-inflammatory properties.
摘要译文
多囊卵巢综合征(PCOS)是最常见的妇科内分泌疾病,会导致无源不育症。当前的研究旨在探索IL-1β抑制剂二蛋素(DIA)在表现出PCOS患者代谢和内分泌标准的大鼠中治疗letrozole诱导的PCOS方面的可能作用。通过口服LeTrozole(1 mg/kg,P.O.)诱导雌性Wistar大鼠PCOS 21天。然后将大鼠用DIA(25 mg/kg/day,P.O.),DIA(50 mg/kg/day,p.o.)或二甲双胍(2 mg/100 g/day,p.o.)治疗,持续14天后。 PCOS导致体重明显更高,卵巢重量,卵巢尺寸和囊肿,以及血清睾丸激素,LH,胰岛素,血糖和脂质剖面水平的升高。 DIA给药可显着降低所有这些影响。此外,DIA明显抑制了卵巢,肌肉和肝脏中letrozole诱导的氧化应激,从而降低了丙二醛和总亚硝酸盐的升高水平,并增加了抑制的超氧化物歧化酶和热酶的抑制水平。 DIA增强了保护蛋白KEAP-1,NRF2和OH-1水平。最后,DIA抑制了NLRP3和caspase-1的mRNA水平升高,上调的炎症细胞因子IL-6,TNF-α和IL-1β/NFκB信号传导途径。我们的结果证明,DIA通过其抗氧化剂和抗炎特性来缓解letrozole诱导的PCOS。
Yasmine F. Ibrahim[a];Mohammed Alorabi[b];Walaa Yehia Abdelzaher[a];Nisreen DM Toni[c];Khaled Thabet[d];AbdelRahman Hegazy[e];Haitham Ahmed Bahaa[e];Gaber El-Saber Batiha[f];Nermeen N. Welson[g];Mohamed A. Morsy[a][h];Katharigatta N. Venugopala[h][i];Asmaa Mohamed Abdel-Aziz[a]. Diacerein ameliorates letrozole-induced polycystic ovarian syndrome in rats[J]. Biomedicine & Pharmacotherapy, 2022,149