摘要
Gamma-Delta (γδ) T cells represent a prominent lymphocyte subset in pigs. Their role and function, however, remains largely unknown. Toll-like receptors (TLR) are key receptors for the recognition of pathogens, but so far, it is unknown if porcine γδ T cells express TLRs and therefore have the innate ability to recognize pathogens through pattern recognition receptors. In this study, we compared γδ T cells in different age groups of pigs and investigated the functional relevance of TLR7/8 expression. We found that the major γδ T cell phenotype shifts from CD2−CD8α−/dimCD27+ in young pigs to CD2+CD8αhighCD27− in 3-year-old pigs impacting their ability to produce IFN-γ upon cytokine and TLR stimulation. Furthermore, we report that stimulation with TLR7/8 ligand R848 increased IFN-γ production in purified γδ T cells upon co-stimulation with IL-2 and IL-12. However, the effect of R848 as a co-activator of γδ T cells was abrogated by the addition of monocytes or within PBMCs, suggesting that γδ T cells respond to multiple direct and indirect stimulations. Thus, our results indicate that γδ T cells express TLRs, are modulated by TLR7/8 ligand R848 and have subset-specific responses.
摘要译文
伽马 - 戴尔塔（γδ）T细胞代表猪中突出的淋巴细胞亚群。但是，它们的作用和功能在很大程度上仍然未知。 Toll样受体（TLR）是识别病原体的关键受体，但到目前为止，尚不清楚猪γδT细胞是否表达TLR，因此具有通过模式识别受体识别病原体的先天能力。在这项研究中，我们比较了猪不同年龄组的γδT细胞，并研究了TLR7/8表达的功能相关性。我们发现，主要的γδT细胞表型从CD2 - cd8α - /DIM cd27 +在CD2 +<<< /sup>cd8α高 cd27 - 在3岁猪中，影响其在细胞因子和TLR刺激下产生IFN-γ的能力。此外，我们报告说，用TLR7/8配体R848刺激在与IL-2和IL-12共刺激后纯化的γδT细胞中增加了IFN-γ的产生。然而，通过添加单核细胞或PBMC中，R848作为γδT细胞的共激活剂的作用被废除了，这表明γδT细胞对多个直接和间接刺激的反应反应。因此，我们的结果表明，γδT细胞表达TLR，由TLR7/8配体R848调节，并具有亚集特异性响应。
Leonie Bettin[a][b];Joseph Darbellay[a];Jill van Kessel[a];Rachelle Buchanan[a];Yurij Popowych[a];Volker Gerdts[a][b]PersonEnvelope. Co-stimulation by TLR7/8 ligand R848 modulates IFN-γ production of porcine γδ T cells in a microenvironment-dependent manner[J]. Developmental & Comparative Immunolog, 2023,138