摘要
PurposeMicrobial fermentation on agro-industrial co-products is an interesting strategy for the bioproduction of metabolites of interest, as it lowers the costs of production and uses renewable and abundant carbon sources. Violacein is a purple pigment with interesting properties that is commonly produced by fermentation. Chromobacterium vaccinii, a wild-type, natural and non-toxic Proteobacteria (Pseudomonadota), has the ability to produce violacein. However, to select an optimal co-product as carbon source, it is necessary to understand its use of the different constituents of plant biomass.MethodsGrowth and violacein production of C. vaccinii were first studied on the main components of plant biomass. Then, they were assessed in presence of raw co-products at various concentrations. Tryptophan, a precursor of violacein biosynthesis, was added in order to evaluate the impact on violacein production yields.ResultsC. vaccinii was able to grow on gluten (wheat protein), as well as on low concentrations of glucose. Selecting protein-rich substrates such as soybean and rapeseed cakes led to improved growth and violacein bioproduction. Addition of tryptophan led to net increases in violacein, but had low impacts on the bacterial growth.ConclusionUnderstanding microbial growth mechanisms during bioproduction of molecules of interest is key in order to select the best adapted agro-industrial co-products used as substrate. This study allowed to better characterize growth of C. vaccinii on various carbon sources and plant biomass, and showed that an optimal substrate with the addition of tryptophan could increase greatly the bioproduction yields of violacein by C. vaccinii.Graphical abstract
摘要译文
对农业工业联合产品的目的性生物发酵是对感兴趣的代谢产物生物生产的有趣策略，因为它降低了生产成本并使用可再生和丰富的碳源。维紫素是一种紫色色素，具有有趣的特性，通常是由发酵产生的。娃娃杆菌是一种野生型，天然和无毒的蛋白杆菌（假单胞菌），具有产生紫leace蛋白的能力。但是，要选择最佳的副产品作为碳源，有必要了解其对植物生物量的不同成分的使用。疫苗生物念珠菌的生生物和紫ack蛋白产生的主要成分首先在植物生物量的主要成分上进行了研究。然后，在存在各种浓度的原始副产品的情况下对它们进行评估。添加了紫紫紫红色生物合成的前体的色氨酸，以评估对紫ciole蛋白产量的影响。 Vaccinii能够在面筋（小麦蛋白）以及低浓度的葡萄糖上生长。选择富含蛋白质的底物，例如大豆和菜籽蛋糕，从而改善了生长和紫roce蛋白生物生产。添加色氨酸会导致紫质蛋白的净增加，但对细菌生长的影响很小。在生物生物生产期间，判断微生物生长机制的感兴趣分子是至关重要的。这项研究可以更好地表征各种碳源和植物生物量在各种碳源和植物生物量上的生长，并表明添加色氨酸的最佳底物可以大大提高C. vaccinii的紫cro摘要。
Cassarini; Mathieu[1];Crônier; David[1];Besaury; Ludovic[1];Rémond; Caroline[1]. Protein-Rich Agro-Industrial Co-products are Key Substrates for Growth of Chromobacterium vaccinii and its Violacein Bioproduction[J]. Waste and Biomass Valorization, 2022,13(11): 4459-4468