摘要
Mastitis is one of the major problems for the productivity of dairy cows and its classifications have usually been based on milk somatic cell counts (SCCs). In this study, we investigated the differences in milk production, rumen fermentation parameters, and diversity and composition of rumen and hindgut bacteria in cows with similar SCCs with the aim to identify whether they can be potential microbial biomarkers to improve the diagnostics of mastitis. A total of 20 dairy cows with SCCs over 500 × 103 cells/mL in milk but without clinical symptoms of mastitis were selected in this study. Random forest modeling revealed that Erysipelotrichaceae UCG 004 and the [Eubacterium] xylanophilum group in the rumen, as well as the Family XIII AD3011 group and Bacteroides in the hindgut, were the most influential candidates as key bacterial markers for differentiating “true” mastitis from cows with high SCCs. Mastitis statuses of 334 dairy cows were evaluated, and 96 in 101 cows with high SCCs were defined as healthy rather than mastitis according to the rumen bacteria. Our findings suggested that bacteria in the rumen and hindgut can be a new approach and provide an opportunity to reduce common errors in the detection of mastitis.
摘要译文
乳腺炎是乳制品奶牛生产率的主要问题之一，其分类通常是基于牛奶体细胞计数（SCC）。在这项研究中，我们调查了奶牛生产，瘤胃发酵参数和多样性和多样性以及具有类似SCCS的奶牛的多样性和组成的差异，目的是识别它们是否可以是潜在的微生物生物标志物，以改善乳腺炎的诊断。在本研究中选择了共有超过500×103个细胞/ ml的SCC的20个乳制品奶牛，但在本研究中选择了乳腺炎的临床症状。随机森林模型显示，瘤胃中的芽孢杆菌004和瘤胃中的XI1-甲基核基团以及在后肠中的乳突XIII ad3011组和菌体，是最有影响力的候选者，作为分化奶牛的“真实”乳腺炎的关键细菌标志物高分SCC。评估了334奶牛的乳腺炎状态，并根据瘤胃细菌定义为101卷的101卷毛牛，而不是瘤胃细菌的乳腺炎。我们的研究结果表明，瘤胃和后肠中的细菌可以是一种新方法，并提供了减少乳腺炎的常见误差的机会。
Yifan Zhong[ 1]; Ming-Yuan Xue[ 1]; Hui-Zeng Sun[ 1]; Teresa G. Valencak[ 1]; Le Luo Guan[ 2];Jianxin Liu[ 1;[*]. Rumen and Hindgut Bacteria Are Potential Indicators for Mastitis of Mid-Lactating Holstein Dairy Cows[J]. Microorganisms, 2020,8(12): 2042