摘要
The consumer market has significantly increased in the search for nutritional and probiotic formulated products. In the present study, probiotic characterization parameters have been evaluated for the bacteria isolated from fermented millet porridge or ragi koozh and jalebi batter. Initially, 30 colonies were randomly picked and screened for lactic acid production. Lactic acid-producing 13 isolates showing gram-positive cell wall, non-spore-forming capacity and catalase-negative strains were subjected to probiotic characterization. Among 13 isolates, 2 isolates S9 and S13 exhibited good tolerance towards in-vitro characterization parameters such as acid and bile tolerance, adhesion to hydrocarbons, autoaggregation, co-aggregation, antibacterial activity, and safety measures. The potential probiotic strains S9 and S13 were identified by molecular methods, and evolutionary imprints and submitted to GenBank as Weisella cibaria and Enterococcus lactis. Results showed the efficacy of probiotic characteristics of isolates S9 and S13 which can be used for the formulation of probiotic products.
摘要译文
在寻找营养和益生菌配方产品时，消费者市场已大大增加。在本研究中，已经评估了从发酵的小米粥或ragi koozh和Jalebi Catter中分离出的细菌的益生菌表征参数。最初，随机采摘30个菌落并筛选乳酸的产生。产生革兰氏阳性细胞壁的产生乳酸的13个分离株，非孢子形成能力和过氧化氢酶阴性菌株受到益生菌的特征。在13个分离株中，有2个分离株S9和S13表现出对体外表征参数的良好耐受性，例如酸和胆汁耐受性，对碳氢化合物的粘附，自身凝集，凝聚，凝聚，抗细菌活性和安全度量。通过分子方法和进化烙印鉴定出潜在的益生菌菌株S9和S13，并以Weisella cibaria和乳酸肠肠球菌的形式提交给Genbank。结果表明，可用于制定益生菌产品的分离株S9和S13的益生菌特征的功效。
Manoharan Manovina[a]PersonEnvelope;Balasubramanian Thamarai Selvi[a];Ragothaman Prathiviraj[b];Joseph Selvin[b]. Potential probiotic properties and molecular identification of lactic acid Bacteria isolated from fermented millet porridge or ragi koozh and jalebi batter[J]. Animal Gene, 2022,26