摘要
A HPLC method for the determination of ergometrine, ergotamine, ergocristine, α-ergocryptine and ergocornine in cereals for animal feed and in mixed feed with high cereal content was developed. Samples were extracted under acidic conditions using a mixture of phosphoric acid and acetonitrile, the extract purified with solid phase extraction cartridges (strong cation exchange), and ergot alkaloids detected after gradient elution on a C18 column by HPLC with fluorescence detection. Detection and determination limits for each individual alkaloid were at 5 (μ/kg and 10 (μg/kg, respectively. With this method, high recovery (82–120%) and good reproducibility was achieved for wheat, rye and mixed feeds, at a sum of total determined alkaloids of < 500 (μg/kg. This method was used to analyse Bavarian feeds (n=124) over three years (2005–2007), and ergot alkaloids were detected in 91 % of the samples. The majority of positive samples had ergot alkaloid contents of < 250 μg/kg, the median alkaloid level was at 70 (μg/kg. The maximum sum of total determined alkaloids exceeded 1000 (μg/kg in wheat, triticale, rye, and mixed feeds, the highest result was obtained for mixed feed (4880 (μg/kg).
摘要译文
高效液相色谱法为麦角新碱，麦角胺，ergocristine的决心，样品使用磷酸和乙腈的混合物在酸性条件下提取，用固相萃取柱纯化的提取物（强阳离子交换），并通过HPLC C18柱荧光检测梯度洗脱之后检测麦角生物碱。每个单独的生物碱的检测和检测限为5（/ kg和10（500（多数阳性样品已经麦角生物碱含量共确定生物碱的最高金额超过1000（
M. Ruhland [1] J. Tischler [1]. Determination of ergot alkaloids in feed by HPLC[J]. Mycotoxin Research, 2008,24(2): 73-79