摘要
Cathelicidins are a family of antibacterial peptides. Human cathelicidin LL-37 inhibits the binding of endotoxin lipopolysaccharide (LPS) to CD14-positive cells and could ameliorate sepsis. The aim of this study was to observe the effect of LL-37 on sepsis in neonatal rats. Intraperitoneal injection (IPI) of LPS was used to create sepsis in suckling rats. Group 1 rats were given LPS with LL-37, group 2 rats were given LL-37 2 h after LPS, and group 3 rats were given LPS without LL-37. Control group rats were given isovolemic normal saline by IPI. Rats given LL-37 IPI were divided into seven subgroups. Following IPI, an overall assessment score (OAS) and rectal temperature (RT) were assessed hourly. Serum C-reactive protein (CRP) was also assessed at death or at sacrifice 10 h after IPI. All rats in group 3 died. For rats receiving lower doses of LL-37 in groups 1 and 2, mortality was decreased. No deaths occurred among those receiving higher doses of LL-37 in group 1; however, mortality increased in group 2. In group 1, OAS and RT deteriorated initially for those receiving lower doses of LL-37, then improved. OAS and RT did not deteriorate throughout the study in rats given higher doses of LL-37. In group 2 rats given higher doses of LL-37, OAS and RT were not significantly different from rats in group 3. CRP was significantly decreased in group 1 compared with group 3, and decreased in group 2 for lower doses only. We conclude that LL-37 may prevent sepsis and be useful in lower doses for treating sepsis. However, LL-37 appears to have adverse effects when used at higher doses for treating sepsis.
摘要译文
Cathelicidins是一个家族抗菌肽。人类抗菌肽LL-37抑制内毒素脂多糖（LPS）的结合到CD14阳性细胞和能改善败血症。本研究的目的是观察的LL-37的上脓毒症新生大鼠的影响。 LPS的腹膜内注射（IPI）用于创建败血症乳鼠。第1组大鼠给予LPS与LL-37，2组大鼠给予LL-37 2小时后LPS，3组大鼠给予LPS不LL-37。通过IPI对照组大鼠给予等容量生理盐水。老鼠给LL-37 IPI被分为七个小组。继IPI，整体评估分数（OAS）和直肠温度（RT）的每小时评估。血清C反应蛋白（CRP）也被评估在死亡或IPI后牺牲10小时。第3组各组大鼠死亡。对于大鼠接受在组1和2更低剂量的LL-37的，死亡率降低。无死亡病例发生在那些在第1组接受高剂量的LL-37;然而，死亡率在组2增加1组，美洲国家组织和RT恶化最初是为那些接受LL-37的低剂量，然后改进。美洲国家组织和RT没有恶化整个研究大鼠给予高剂量的LL-37。在第2组大鼠给予高剂量的LL-37，美洲国家组织和RT都不在组3 CRP在第1组显著下降与3组比较显著不同的大鼠，和降低的第2组仅较低剂量。我们的结论是LL-37可以防止败血症和在更低的剂量用于治疗败血症是有用的。然而，LL-37似乎具有不利的影响，在高剂量的用于治疗败血症使用时。
Koji Fukumoto [1] Isao Nagaoka [2] Atsuyuki Yamataka [1] Hiroyuki Kobayashi [1] Toshihiro Yanai [1] Yoshifumi Kato [1] Takeshi Miyano [1]. Effect of antibacterial cathelicidin peptide CAP18/LL-37 on sepsis in neonatal rats[J]. Pediatric Surgery International, 2005,21(1): 20-24