摘要
BackgroundRhizopus species is among the most well-known lipase producers, and its enzyme is suitable for use in many industrial applications. Our research focuses on the production of lipase utilizing waste besides evaluating its applications.ResultsAn extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The partially purified enzyme was non-specific lipase and the utmost activity was recorded at pH 6, 40 °C with high stability for 30 min. The constants Km and Vmax, calculated from the Lineweaver-Burk plot, are 0.3 mg/mL and 208.3 U/mL, respectively. Monovalent metal ions such as Na+ (1 and 5 mM) and K+ (5 mM) were promoters of the lipase to enhance its activity with 110, 105.5, and 106.5%, respectively. Chitosan was used as a perfect support for immobilization via both adsorption and cross-linking in which the latter method attained immobilization efficiency of 99.1% and reusability of 12 cycles. The partially purified enzyme proved its ability in forming methyl oleate (biodiesel) through the esterification of oleic acid and transesterification of olive oil.ConclusionThe partially purified and immobilized lipase from Rhizopus oryzae R1 approved excellent efficiency, reusability, and a remarkable role in detergents and biodiesel production.
摘要译文
BackdactRhizopus物种是最著名的脂肪酶生产商之一，其酶适用于许多工业应用。我们的研究重点是利用废物的生产，除了评估其应用外，还要通过硫酸氨降水量从根茎oryzae r1孤立的根茎oryzae r1孤立的培养汤中部分纯化细胞外脂肪酶，然后通过透析脱水。部分纯化的酶是非特异性脂肪酶，最大活性在6，40°C下记录，高稳定性30分钟。从线条框架图计算得出的常数K M和V Max分别为0.3 mg/ml和208.3 U/ml。单价金属离子，例如Na +（1和5 mm）和K +（5 mm）是脂肪酶的启动子，以增强其活性，以110、105.5和106.5和106.5 ％， 分别。通过吸附和交联，壳聚糖被用作固定化的完美支持，其中后一种方法达到了99.1％的固定效率和12个循环的可重复性。部分纯化的酶通过酯化油酸的酯化和橄榄油的静脉化来证明其能够形成甲基油蛋白（生物柴油）。判断，从根茎oryzae oryzae r1批准的良好的效率，可重复性和脱水剂和生物埃尔德式埃尔德柴油埃尔德柴油的作用中，部分纯化和固定的脂肪酶从生产。
Helal; Shimaa E.[1];Abdelhady; Hemmat M.[2];Abou-Taleb; Khadiga A.[2];Hassan; Mervat G.[1];Amer; Mahmoud M.[1]. Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production[J]. Journal of Genetic Engineering and Biotechnology, 2021,19: 1-13