摘要
In recent years, whole-plant corn silage has been widely used in China. Roughage is an important source of nutrition for ruminants and has an important effect on rumen microbiota, which plays an important role in animal growth performance and feed digestion. To better understand the effects of different silages on rumen microbiota, the effects of whole-plant corn silage or corn straw silage on growth performance, rumen fermentation products, and rumen microbiota of Simmental hybrid cattle were studied. Sixty healthy Simmental hybrid cattle were randomly divided into 2 groups with 6 replicates in each group and 5 cattle in each replicate. They were fed with whole-plant corn silage (WS) diet and corn straw silage (CS) diet respectively. Compared with corn straw silage, whole-plant corn silage significantly increased daily gain and decreased the feed intake-to-weight gain ratio (F/G) of beef cattle. Whole-plant corn silage also decreased the acetic acid in the rumen and the acetate-to-propionate ratio (A/P) compared with corn straw silage. On the genus level, the relative abundance of Prevotella_1 was significantly increased while the relative abundance of Succinivibrionaceae_UCG-002 was decreased in cattle fed whole-plant corn silage compared with those fed corn straw silage. Prevotella_1 was positively correlated with acetic acid and A/P. Succinivibrionaceae_UCG-002 was positively correlated with propionic acid and butyric acid, and negatively correlated with pH. Feeding whole-plant corn silage improved amino acid metabolism, nucleotide metabolism, and carbohydrate metabolism. Correlation analysis between rumen microbiota and metabolic pathways showed that Succinivibrionaceae_UCG-002 was negatively correlated with glycan biosynthesis and metabolism, metabolism of co-factors and vitamins, nucleotide metabolism, and translation while Prevotellaceae_UCG-003 was positively correlated with amino acid metabolism, carbohydrate metabolism, energy metabolism, genetic information processing, lipid metabolism, membrane transport, metabolism of cofactors and vitamins, nucleotide metabolism, replication and repair, and translation. Ruminococcus_2 was positively correlated with amino acid metabolism and carbohydrate metabolism. Feeding whole-plant corn silage can improve the growth performance and rumen fermentation of beef cattle by altering rumen microbiota and regulating the metabolism of amino acids, carbohydrates, and nucleotides.Key points• Feeding whole-plant corn silage could decrease the F/G of beef cattle• Feeding whole-plant corn silage improves rumen fermentation in beef cattle• Growth performance of beef cattle is related to rumen microbiota and metabolism
摘要译文
近年来，全植物玉米青贮饲料在中国被广泛使用。粗糙是反刍动物的重要营养来源，对瘤胃微生物群具有重要作用，瘤胃微生物群在动物生长表现和饲料消化中起着重要作用。为了更好地了解不同青贮饲料对瘤胃微生物群的影响，研究了整个植物玉米青贮饲料或玉米秸秆青贮饲料对生长性能，瘤胃发酵产物和象征杂种牛的瘤胃菌群的影响。将60个健康的模拟杂种牛随机分为2组，每组中有6个重复，每组重复5个牛。他们分别以全植物玉米青贮饲料（WS）饮食和玉米稻草青贮饲料（CS）饮食喂食。与玉米稻草青贮饲料相比，全植物玉米青贮饲料显着增加了每日增益，并降低了肉牛的进食摄入量增长比（F/G）。与玉米稻草青贮饲料相比，全植物玉米青贮饲料还降低了瘤胃中的乙酸和乙酸酯与丙酸酸酯比（A/P）。在属水平上，与那些饲养的玉米稻草青贮饲料相比，在饲喂全植物玉米灰青贮饲料的牛中，琥珀酰基二酸_ucg-002的相对丰度显着增加，而琥珀酰亚胺科的相对丰度减少了。 Prevotella_1与乙酸和A/P呈正相关。琥珀酰氨基酸-ucg-002与丙酸和丁酸呈正相关，并且与pH值负相关。喂养全植物玉米青贮饲料改善了氨基酸的代谢，核苷酸代谢和碳水化合物代谢。 Correlation analysis between rumen microbiota and metabolic pathways showed that Succinivibrionaceae_UCG-002 was negatively correlated with glycan biosynthesis and metabolism, metabolism of co-factors and vitamins, nucleotide metabolism, and translation while Prevotellaceae_UCG-003 was positively correlated with amino acid metabolism, carbohydrate metabolism,能量代谢，遗传信息加工，脂质代谢，膜转运，辅因子和维生素的代谢，核苷酸代谢，复制和修复以及翻译。 Ruminoccus_2与氨基酸代谢和碳水化合物代谢呈正相关。通过改变瘤胃微生物群，调节氨基酸，碳水化合物和核苷酸的代谢，可以改善肉牛的生长性能和瘤胃发酵。Keyke点•喂食全植物玉米灰累可能会降低氨基酸的代谢。牛肉•喂食全植物玉米青贮饲料改善肉牛瘤瘤牛妈妈的发酵•牛牛的生长表现与瘤胃微生物群和代谢有关
Cui; Yalei[1];Liu; Hua[1];Gao; Zimin[1];Xu; Junying[1];Liu; Boshuai[1];Guo; Ming[1];Yang; Xu[1];Niu; Jiakuan[1];Zhu; Xiaoyan[1];Ma; Sen[1];Li; Defeng[1];Sun; Yu[1];Shi; Yinghua[1]. Whole-plant corn silage improves rumen fermentation and growth performance of beef cattle by altering rumen microbiota[J]. Applied Microbiology and Biotechnology, 2022,106(11): 4187-4198