摘要
The aim of the present study was to investigate the in vitro antioxidant potential of the cell-free extracts (CFE) of two probiotic bacteria Bacillus amyloliquefaciens ssp. plantarum IMV B-7142 and Bacillus amyloliquefaciens ssp. plantarum IMV B-7143 and their hepatoprotective effects. These strains are the main components of the veterinary probiotic preparation endosporyn. The CFE of probiotic bacteria were able to stabilize the 2.2-diphenyl-1-picrylhydrazyl radical to its neutral form at their cultivation during 24–48 h. But this index was more pronounced for the IMV B-7142 strain and amounted to 44.4–51.2%. The hydroxyl radical scavenging activity of the CFE of probiotic bacteria increased more than 70–80% regardless of the cultivation period (24–48 h). The antioxidant potential of probiotic strains is associated with the synthesis of the multiple biologically active molecules. The phenolic and benzoic acids-antioxidants (gallic, 4-hydroxyphenylacetic, caffeic, syringic, p-coumaric, trans-ferulic, and trans-cinnamic acids) were identified among metabolites of B. amyloliquefaciens ssp. plantarum strains. The CFE of probiotic strains were able to protect of rat hepatocytes from the toxic effects of the carbon tetrachloride (CCl4). Post-treatment of stress-induced rat hepatocytes by CFE of the IMV B-7042 was accompanied by an increase of the catalase activity of cells by 485.2 mM/min × mg of protein, compared to stress-damaged sample. In doing so, the content of the main markers of oxidative stress: lipid hydroperoxides and malondialdehyde decreased significantly. The results suggested that CFE of both probiotic strains have potent antioxidant properties and effectively protect of stress-damaged rat hepatocytes.
摘要译文
本研究的目的是探讨两种益生菌细菌芽孢杆菌SSP的无细胞提取物（CFE）的体外抗氧化潜力。 Plantarum IMV B-7142和Bacillus淀粉醇溶液SSP。 plantarum imv b-7143及其肝脏保护作用。这些菌株是兽医益生菌制剂的主要组成部分。益生菌细菌的CFE能够在24-48小时期间在它们的培养时稳定2.2-二苯基-1-富铬酰基的其中性形式。但该指数对IMV B-7142菌株更加明显，达到44.4-51.2％。无论培养期（24-48小时），益生菌细菌CFE的羟基自由基清除活性增加了70-80％。益生菌菌株的抗氧化潜力与多种生物活性分子的合成有关。在B.淀粉醇提取物SSP的代谢物中鉴定了酚类和苯甲酸 - 抗氧化剂（Gallic，4-羟基苯基，冠状动脉，注射剂，对生糖尿病和转肉酸）。 Plantarum菌株。益生菌菌株的CFE能够保护大鼠肝细胞免受四氯化碳（CCl 4）的毒性作用。与应力损坏的样品相比，IMV B-7042的CFE通过CFE的CFE的肝细胞的后处理伴随着485.2mm / min×mg蛋白质的含量增加。在这样做时，氧化应激的主要标志物的含量：脂质氢过氧化物和丙二醛显着降低。结果表明，益生菌菌株的CFE具有有效的抗氧化特性，有效地保护应力受损大鼠肝细胞。
Safronova; L. S.[1];Skorochod; I. A.[1];Ilyash; V. M.[1]. Antioxidant and Antiradical Properties of Probiotic Strains Bacillus amyloliquefaciens ssp. plantarum[J]. Probiotics and Antimicrobial Proteins, 2021,13(6): 1585-1597