摘要
A 7-week growth trial was conducted to assess the potential of using soybean protein isolate (SPI) to replace poultry by-product meal (PBM) protein in low fishmeal (FM) diets for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). Seven isoproteic (490 g kg−1) and isolipidic (90 g kg−1) experimental diets were formulated, including six low FM diets and a high FM diet (HFMP). The low FM diets contained 0, 74.8, 149.7, 224.5, 299.4 and 374.2 g kg−1 SPI, replacing approximately 0%, 20%, 40%, 60%, 80% and 100% of the PBM protein (designated as SPIP0, SPIP20, SPIP40, SPIP60, SPIP80 and SPIP100, respectively). Each diet was hand-fed to triplicate groups of 12 hybrid grouper juveniles (average initial body weight: 14.30 ± 0.1 g per fish). After being fed for 7 weeks, fish fed with HFMP and SPIP60 showed significantly higher (p < .01) weight gain percentage (WG%), protein efficiency ratio and protein productive value than fish fed with other experimental diets. These three parameters were elevated with the increment in PBM protein (PBMP) replacements, reaching the peak value at the 60% replacing level, and then, they were significantly reduced at the 80% or 100% replacing level. Analysis of the two-slope straight broken-line model showed that the maximum PBMP replacement level by SPI protein (SPIP) at the intercept value of WG% was estimated to be 60%. Feed conversion ratio showed an opposite tendency of variation to WG%. Fish fed with SPIP80 and SPIP100 displayed lower mRNA levels of hepatic insulin-like growth factor-1, hypothalamus neuropeptide Y and head-kidney nuclear factor erythroid 2-related factor 2 than fish fed with other experimental diets. Generally, 60% of PBM protein could be replaced with SPI protein in low FM diets for hybrid grouper.
摘要译文
进行了7周的增长试验，以评估使用大豆蛋白分离物（SPI）的潜力替代少年杂交石榴石的低鱼粉（FM）饮食中的家禽副产品膳食（PBM）蛋白（Epinephelus fuscoguttatus♀×epinephelus lanceolatus♂ ）。制定了七种纯（490g kg -1-1）和溶素酸（90g kg -1-1）实验饮食，包括六种低fm饮食和高fm饮食（hfmp） 。低FM饮食含有0,74.8,149.7,224.5,299.4和374.2g kg -1 -1 spi，取代约0％，20％，40％，60％，80％和100％ PBM蛋白（分别指定为SPIP0，SPIP20，SPIP40，SPIP60，SPIP80和SPIP100）。每次饮食都被送入12份杂交石榴幼虫（平均初始体重）：每条鱼的平均初始体重：14.30±0.1g）。饲喂7周后，用HFMP和SPIP60喂养的鱼显着更高（P <.01）重量增加百分比（WG％），蛋白质效率比和蛋白质生产价值，而不是饲喂其他实验饮食。这三个参数随着PBM蛋白（PBMP）替代的增量升高，达到60％的替代水平的峰值，然后，在80％或100％的更换水平下它们显着降低。双坡直线模型的分析表明，WG％截距值的SPI蛋白（SPIP）的最大PBMP替代水平估计为60％。饲料转化率显示出与WG％变异的相反趋势。用Spip80和Spip100喂养的鱼显示肝胰岛素样生长因子-1，下丘脑神经肽Y和头肾核因子红细胞2相关因子2的肝脏胰岛素样的较低的mRNA水平而不是饲喂其他实验饮食的鱼。通常，60％的PBM蛋白质可以以低FM饮食中的SPI蛋白替换为杂种石斑鱼。
Ye Bo[1];Xue Min[2];Wu Xiufeng[2];Wu Xiaoyi[1];Wang Xiao[1];Ma Lei[1];Mu Wei[1];Geng Lina[1];Cai Qinxiao[1];Zhang Lu[1];Zhai Haoyun[1];Zhou Zhiyu[1];Misbah Irm[1]. Replacing poultry by-product meal protein with soybean protein isolate in low fishmeal diets for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)[J]. Aquaculture Nutrition, 2021,27(6): 2405-2415