摘要
Trans-fatty acids (TFAs) consumption has created concerns regarding male/female reproductive system. However, the effects of TFA in paternal diet on offspring's reproduction have not been addressed. The purpose of this study is to investigate the effects of rat paternal TFAs and vitamin E consumption on offspring's sperm quality and expression pattern of peroxisome proliferator-activated receptors (PPARs) in testis tissues. Forty adult male rats were randomly divided into four groups: Control diet (C); Control diet plus TFA (CTH); diet supplemented with vitamin E (E) and a diet containing vitamin E and TFA (ETH). Mother rats had normal diet during gestation period. Three offspring from each group were chosen randomly and their testicular samples were collected, and sperm parameters were measured by CASA. Our results indicate that feeding fathers with TFA can negatively affect offspring's sperm concentration and motility, while consumption of vitamin E can improve these parameters (p PPARβ and PPARγ genes, whereas vitamin E-containing diet up-regulated the transcription of PPAR genes. In conclusion, TFA intake in paternal diet may have negative effects on reproductive system of the offspring while vitamin E may not diminish these effects.
摘要译文
反式脂肪酸（TFAS）消费为男性/女性生殖系统创造了担忧。然而，尚未解决TFA在后代繁殖中饮食的影响。本研究的目的是探讨大鼠父母TFAS和维生素E消费对睾丸组织中过氧化物激素活化受体（PPAR）的后代精子质量和表达模式的影响。四十个成年雄性大鼠随机分为四组：控制饮食（C）;控制饮食加TFA（CTH）;饮食补充有维生素E（e）和含有维生素E和TFA（Eth）的饮食。母亲大鼠在妊娠期期间有正常的饮食。随机选择从每组的三个后代，并收集它们的睾丸样品，并通过CASA测量精子参数。我们的研究结果表明，与TFA的饲喂父亲可以对后代的精子浓度和动力产生负面影响，而维生素E的消耗可以改善这些参数（PPARβ和PPARγ基因，而维生素E的饮食上调PPAR基因的转录。总之，患者饮食中的TFA摄入可能对后代的生殖系统产生负面影响，而维生素E可能无法减少这些效果。
Narges Khamehiee[1];Parham Jazireian[2];Bita Ebrahimi[3];AliReza Alizadeh[3];Maryam Shahhoseini[2]. Paternal trans-fatty acid and vitamin E diet affects rat offspring's semen quality and PPARs expression[J]. Andrologia, 2021,53(7)