摘要
This study compared differential concentrations of chicken egg yolk (v/v; T1, 10%; T2, 15%; and T3, 20%) in commercial extender Triladyl® with tris-citric acid based extender (v/v; chicken egg yolk: 20%; C, control) for improving the in vitro quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa at post–thawing. The mean values of progressive motility (PM, %), rapid velocity (RV, %), average path velocity (VAP, μm sec−1), straight line velocity (VSL, μm sec−1), curvilinear velocity (VCL, μm sec−1), supra-vital plasma membrane integrity (SV−PMI, %), mitochondrial transmembrane potential (MMP, %), and viable with intact acrosome (V−IACR, %) were significantly higher(except significantly lower medium velocity; MV, %)in T1 and T2with than control at all stages. Mean values of straightness (STR – VSL: VAP %) and linearity (LIN – VSL: VCL %) were significantly higher in T1 and T2 than control at PD. In vitro longevity, from 0 to 3 h incubation (37 °C), showed significantly low PM (T1) and RV (T1 and T2) percentage loss than control at PT. A significantly positive Pearson’s correlation was noticed between CASA and SV−PMI, MMP, V−IACR, and DNA integrity (%). Significantly higher in vivo fertilization rates (%) were recorded in T1 (73.53) and T2 (74.19) compared to the C (46.88) and T3 (48.28), respectively. In conclusion, addition of even chicken egg yolk at 10% (v/v) in Triladyl® improves the cryopreserved in vitro and in vivo quality of Beetal buck spermatozoa as compared to tris-citric acid extender with 20% (v/v) egg yolk.
摘要译文
这项研究比较了鸡蛋蛋黄的差异浓度（v/v; t1，10％; t2，15％; t3; t3，20％）与基于Tris-citric Acid的扩展器（v/v;鸡蛋蛋黄：20％; c，对照）用于改善甲基雄鹿（Capra hircus）精子的体外质量和体内受精的潜力。进行性运动的平均值（PM，％），快速速度（RV，％），平均路径速度（VAP，μmsec -1 ），直线速度（VSL，VSL，μmsec -1 ），曲线速度（VCL，μmsec -1 ），上质量质膜膜完整性（SV-PMI，％），线粒体式跨膜脑电位（MMP，％）和％）以及在T1和T2With中，具有完整的顶体（V -IACR，％）的可行性（V -IACR，％）明显更高（除外，MV，MV，％）比所有阶段都高于对照。T1和T2的平均直度（STR - VSL：VSL：VSL％）和线性（LIN - VSL：VCL％）的平均值明显高于PD的对照。从0到3 h孵育（37°C）的体外寿命显示出明显较低的PM（T1）和RV（T1和T2）百分比损失的百分比损失了。在CASA和SV -PMI，MMP，V -IACR和DNA完整性（％）之间，注意到了Pearson的显着正相关。与C（46.88）和T3（48.28）相比，在T1（73.53）和T2（74.19）中，体内受精率（％）明显更高。总之，与Tris-citric-citric Acid Expenender相比，在Triladyl®中以10％（v/v）添加鸡蛋蛋黄（v/v）可改善甲虫雄鹿精子的冷冻保存和体内质量。蛋黄。
Syed Aftab Hussain Shah; Muhammad Shafiq Haider; Hussain Ahmed; Muhammad Hammad Fayyaz; Syed Murtaza Hassan Andrabi. Triladyl® improves the cryopreserved quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa[J]. Small Ruminant Research, 2023,221