摘要
To identify differences in rumen function as a result of feeding monensin to beef cattle, rumen fluid metagenomics and metabolomics analyses were used to evaluate the functional attributes and metabolites of rumen microbiota in beef steers fed no or 200 mg/d of monensin. Eight rumen-fistulated steers were used in the study for a period of 53 days. Rumen fluid samples were collected on the last day of the experiment. Monensin increased the relative abundance of Selenomonas sp. ND2010, Prevotella dentalis, Hallella seregens, Parabacteroides distasonis, Propionispira raffinosivorans, and Prevotella brevis, but reduced the relative abundance of Robinsoniella sp. KNHs210, Butyrivibrio proteoclasticus, Clostridium botulinum, Clostridium symbiosum, Burkholderia sp. LMG29324, and Clostridium butyricum. Monensin increased the relative abundance of functional genes involved in amino acid metabolism and lipid metabolism. A total of 245 metabolites were identified. Thirty-one metabolites were found to be differentially expressed. Pathway analysis of the differentially expressed metabolites revealed upregulated metabolic pathways associated with metabolism of linoleic acid and some amino acids. These findings confirm that monensin affects rumen fermentation of forage-fed beef cattle by modulating the rumen microbiome, and by reducing amino acid degradation and biohydrogenation of linoleic acid in the rumen.
摘要译文
为了鉴定萌发宫蛋白的蛋白荧光蛋白患者的差异，用于评估饲喂NO或200mg / d Monensin的牛肉带中瘤胃微生物的功能属性和代谢物。在研究中使用八个瘤胃瘘管53天。在实验的最后一天收集瘤胃液样品。宫蛋白增加了Selenomonas sp的相对丰度。 ND2010，Fvovotella Dentalis，Hallella Seregens，Paramacteroides Distasonis，Propionispira roffinosivorans和Fvootella Brevis，但减少了Robinsoniella SP的相对丰富。 Knhs210，抑虫剂Proteoclasticus，Clostridium botulinum，Clostridium symbosum，Burkholderia sp。 LMG29324和Clostridium Butricum。宫蛋白增加了氨基酸代谢和脂质代谢参与的相对丰富的官能基因。共鉴定了总共245个代谢物。发现三十一体代谢物被差异表达。差异表达代谢物的途径分析显示出与亚油酸和一些氨基酸的代谢相关的上调的代谢途径。这些发现证实，宫蛋白通过调节瘤胃微生物组，并通过降低瘤胃中亚油酸的氨基酸降解和生物中的氨基酸降解和生物中水来影响饲料喂食牛肉的瘤胃发酵。
Ibukun Ogunade[ [*]; Hank Schweickart; Kenneth Andries; Jerusha Lay;James Adeyemi. Monensin Alters the Functional and Metabolomic Profile of Rumen Microbiota in Beef Cattle[J]. Animals, 2018,8(11): 211