摘要
This study aimed to evaluate the effects of fermented corn–soybean meal mixed feed on intestinal barrier function and cecal microbiota in laying hens. A total of 360 Jingfen No.6 laying hens (22 wk-old) were assigned to 4 dietary treatments, which were offered basal diets (without antibiotics) containing 0, 4, 6 and 8% of fermented mixed feed respectively. The results showed that the pH value and anti-nutritional factor concentrations in fermented mixed feed were lower than those in unfermented feed (p < 0.05). Moreover, fermentation in the feed significantly increased the crude protein content (p < 0.05). Supplementation with fermented feed significantly reduced the crypt depth and increased the villi height:crypt depth ratio of duodenum and jejunum (p < 0.05). Meanwhile, fermented feed increased the secretory immunoglobulin A content and MUC2 mRNA expression of jejunum (p < 0.05). These beneficial effects were exhibited at the addition level ≥6% and microbial composition of caeca in the control, and so 6% fermented feed groups were analyzed. The structure of the gut microbiota was remarkably altered by additions, characterized by increased abundances of some health-promoting bacteria, such as Parasutterella, Butyricicoccus and Erysipelotrichaceae (p < 0.05). In summary, fermented mixed feed modulated cecal flora, subsequently contributing to improvements in intestinal morphology and barrier functions in laying hens.
摘要译文
本研究旨在评估发酵玉米豆粕混合饲料对饲养母鸡肠道屏障功能和宫颈微生物的影响。共有360号铺设母鸡（22周龄）（22周龄）分配到4个饮食治疗，其中分别提供含有0,4,6和8％的发酵混合饲料的基础饮食（无抗生素）。结果表明，发酵混合饲料中的pH值和抗营养因子浓度低于未加入的饲料（P <0.05）。此外，饲料中的发酵显着增加了粗蛋白质含量（P <0.05）。发酵饲料的补充显着降低了隐窝深度并增加了绒毛高度：Duodenum和Jejunum的Crypt Degress比率（P <0.05）。同时，发酵饲料增加了分泌物免疫球蛋白的含量和Muc2 mRNA表达的Jejunum（P <0.05）。在对照中添加了这些有益效果，并在对照中的CAECA的微生物组合物中表现出来，因此分析了6％发酵进料组。通过添加显着改变肠道微生物的结构，其特征在于一些健康促进细菌的丰度，例如Parasutrella，丁基（Butyricoccus和Erysipelotrichaceae）（P <0.05）。总之，发酵的混合饲料调节的盲肠菌群，随后有助于改善饲养母鸡的肠形态和屏障功能。
Yinglu Liu;Jia Feng;Yamin Wang;Jing Lv;Jinghe Li;Lijuan Guo;Yuna Min. Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens[J]. Animals, 2021,11(11): 3059