摘要
BackgroundIntestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on feed efficiency of livestock animals. However, the intestinal microbes that are critically involved in feed efficiency remain elusive.MethodsTo identify intestinal bacteria associated with residual feed intake (RFI) in chickens, male Cobb broiler chicks were individually housed from day 14 to day 35. Individual RFI values were calculated for 56 chickens. Luminal contents were collected from the ileum, cecum, and cloaca of each animal on day 35. Bacterial DNA was isolated and subjected to 16S rRNA gene sequencing. Intestinal microbiota was classified to the feature level using Deblur and QIIME 2. High and low RFI groups were formed by selecting 15 and 17 chickens with the most extreme RFI values for subsequent LEfSe comparison of the difference in the microbiota. Spearman correlation analysis was further performed to identify correlations between the intestinal microbiota composition and RFI.ResultsNo significant difference in evenness, richness, and overall diversity of the microbiota in the ileum, cecum, or cloaca was observed between high and low RFI chickens. However, LEfSe analysis revealed a number of bacterial features being differentially enriched in either high or low RFI chickens. Spearman correlation analysis further identified many differentially enriched bacterial features to be significantly correlated with RFI (P < 0.05). Importantly, not all short-chain fatty acid (SCFA) producers showed a positive association with RFI. While two novel members of Oscillibacter and Butyricicoccus were more abundant in low-RFI, high-efficiency chickens, several other SCFA producers such as Subdoligranulum variabile and two related Peptostreptococcaceae members were negatively associated with feed efficiency. Moreover, a few closely-related Lachnospiraceae family members showed a positive correlation with feed efficiency, while others of the same family displayed an opposite relationship.ConclusionsOur results highlight the complexity of the intestinal microbiota and a need to differentiate the bacteria to the species, subspecies, and even strain levels in order to reveal their true association with feed efficiency. Identification of RFI-associated bacteria provides important leads to manipulate the intestinal microbiota for improving production efficiency, profitability, and sustainability of poultry production.
摘要译文
背景肠道菌群在营养物质的消化和利用中起着关键作用，对牲畜的饲料效率产生深远的影响。然而，与饲料效率密切相关的肠道微生物仍然难以捉摸。方法为了鉴定与鸡剩余饲料摄入量（RFI）相关的肠道细菌，从第14天到第35天分别饲养雄性Cobb肉鸡。 56只鸡。在第35天从每只动物的回肠，盲肠和泄殖腔收集发光物质。分离细菌DNA，并进行16S rRNA基因测序。使用Deblur和QIIME 2将肠道菌群分类为特征水平。通过选择15和17只具有最高RFI值的鸡来形成高和低RFI组，以便随后对微生物群差异进行LEfSe比较。进一步进行Spearman相关分析以鉴定肠道菌群组成与RFI之间的相关性。结果在高和低RFI鸡之间，回肠，盲肠或泄殖腔中菌群的均匀度，丰富度和总体多样性没有显着差异。但是，LEfSe分析显示，在高或低RFI鸡中，许多细菌特征差异富集。 Spearman相关分析进一步确定了许多差异丰富的细菌特征与RFI显着相关（P <0.05）。重要的是，并非所有的短链脂肪酸（SCFA）生产商都与RFI呈正相关。在低RFI，高效率的鸡中，Oscillibacter和Butyricicoccus的两个新颖成员更为丰富，而其他几个SCFA生产者（如变亚肉芽孢杆菌）和两个相关的Peptostreptococcaceae成员则与饲料效率负相关。此外，一些紧密相关的鞭毛藻科成员与饲料效率呈正相关，而同一家族的其他成员则呈相反的关系。结论我们的结果强调了肠道菌群的复杂性，以及将细菌区分为物种，亚种的需求。 ，甚至应变水平，以揭示它们与饲料效率的真正关联。 RFI相关细菌的鉴定为操纵肠道菌群提供了重要线索，从而提高了家禽生产的生产效率，盈利能力和可持续性。
Jing Liu[1];Sydney N. Stewart[1];Kelsy Robinson[1];Qing Yang[1];Wentao Lyu[1];Melanie A. Whitmore[1];Guolong Zhang[1]. Linkage between the intestinal microbiota and residual feed intake in broiler chickens[J]. Journal of Animal Science and Biotechnology, 2021,12(1)