摘要
Compounds of the cell walls of heat-killed lactic acid bacteria show immunomodulatory properties which boost immunological systems, and are used ad postbiotics (paraprobiotics). In this study, we used 17 different heat-killed isolates as postbiotics and evaluated their anti-inflammatory potential on the expression of proinflammatory mediators and cellular signaling pathways of murine macrophage, RAW 264.7 cells. Bifidobacterium bifidum MG731 showed the high 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (90.6%), followed by Bifidobacterium lactis MG741 (59.6%). The Bi. lactis MG741 showed the high ABTS free radical scavenging activity (99.5%), followed by Lactobacillus plantarum MG989 (98.9%), Lactobacillus salivarius MG242 (97.1%), and Bi. bifidum MG731 (96.1%). In addition, Bi. bifidum MG731 showed the lowest nitric oxide production (4.28 μM), followed by B. lactis MG741 (10.80 μM), L. salivarius MG242 (14.60 μM), and L. plantarum MG989 (19.60 μM). The selected strains showed a decreased nitric oxide production via downregulation of inducible nitric oxide synthase and cyclooxygenase 2, which were upregulated via LPS-stimulated RAW 264.7 macrophages. Short-chain fatty acids (SCFA) including acetic, propionic, and butyric acid were produced by four strains. The Bi. bifidum MG731 showed total SCFAs production (4998.6 μg/g), Bi. lactis MG741 (2613.9 μg/g), L. salivarius MG242 (1456.1 μg/g), and L. plantarum MG989 (630.2 μg/g). These results indicated that the various selected strains may possess an anti-inflammatory potential and provide a molecular basis for the development of functional probiotics.
摘要译文
热杀死的乳酸菌细胞壁的化合物显示出免疫系统的免疫调节性能，并使用过Poctbiotics（Paraprobiotics）。在这项研究中，我们使用17种不同的热杀死的分离物作为后期生物质，并评估它们对鼠巨噬细胞的促炎介质和细胞信号通路的表达的抗炎潜力，生成的264.7细胞。双歧杆菌Mg731显示出高2,2-二苯基-1-富铬酰基（DPPH）自由基清除活性（90.6％），其次是双歧杆菌MG741（59.6％）。伯。 Lactis Mg741显示出自由基自由基清除活性（99.5％），其次是Lactobacillus Mg989（98.9％），乳杆菌唾液Mg242（97.1％）和Bi。 Bifidum mg731（96.1％）。此外，BI。 Bifidum Mg731显示了最低的一氧化氮产生（4.28μm），其次是B.乳酸Mg741（10.80μm），L.唾液Mg242（14.60μm）和L.Purerarum mg989（19.60μm）。所选择的菌株通过诱导型一氧化氮合酶和环加氧酶2的下调显示一氧化氮产生，其通过LPS刺激的原料264.7巨噬细胞上调。包括醋酸，丙种和丁酸的短链脂肪酸（SCFA）由四种菌株产生。伯。 Bifidum Mg731显示了SCFA总产量（4998.6μg/ g），BI。 Lactis Mg741（2613.9μg/ g），L. salivarius mg242（1456.1μg/ g）和l. plantarum mg989（630.2μg/ g）。这些结果表明，各种选定的菌株可具有抗炎潜力，并为功能性益生菌的发育提供分子基础。
Chang-Ho Kang;Jin-Seong Kim;Nam-Soo Paek;. Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods[J]. 3 Biotech, 2021,11(5): 1-8