摘要
BackgroundIdentification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry. Concentrations of the small peptide hormone oxytocin (OXT), involved in male reproductive function and present in the seminal plasma (SP) of several species could be a robust one. This study characterized concentrations of SP-OXT in ejaculates from boars used in artificial insemination (AI) programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored AI-semen. Seminal OXT concentrations (ng/mL) were measured in 169 ejaculates from 61 boars of the Duroc, Pietrain, Landrace and Large White breeds using a direct competitive immunoassay test based on AlphaLISA® technology. Ejaculate (ejaculate volume, sperm concentration, total sperm count) and sperm parameters (motility, viability, intracellular generation of reactive oxygen species, plasma membrane fluidity) were assessed at 0 h and 72 h in AI-semen samples stored at 17 °C. In vivo fertility included only 18 Large White and Landrace boars whose AI-semen was used to inseminated > 100 sows and evaluated both farrowing rate and litter size of 3,167 sows.ResultsThe results showed that SP-OXT differed between boars and between ejaculates within boar (P < 0.05) but not between breeds (Duroc, Pietrain, Landrace and Large White). Ejaculates with higher SP-OXT concentration/mL (hierarchically grouped; P < 0.001) had larger volume and came from younger boars (P < 0.05). Ejaculates of boars showing positive farrowing rate deviation exhibited higher (P < 0.05) SP-OXT concentration/mL than those with negative farrowing rate deviation.ConclusionThe SP concentrations of OXT are boar, ejaculate and age dependent, and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.
摘要译文
在体生物标记生育相关BackgroundIdentification仍然是畜牧业是一个挑战。小肽激素催产素（OXT）的浓度，涉及男性生殖功能和本几个物种的精浆（SP）中可能是一个强大的一个。这项研究中，其特征在于SP-OXT的浓度在从精液中人工授精（AI）程序所使用的公猪旨在评价其与精子质量变量的关系和在体内生育它们的液体存储的AI-精液。精液OXT浓度（毫微克/毫升）在从杜洛克，皮特兰，长白猪和大白猪品种的61种公猪精液169使用基于的AlphaLISA ®技术的直接竞争性免疫试验来测定。射精（射精量，精子浓度，总精子数）和精子参数（运动性，活力，细胞内产生活性氧，血浆膜流动性），在0小时和72小时储存在17℃下AI-精液样品中进行了评估。体内生育只包括18大白猪和长白猪公猪其AI-精液用于授精> 100头母猪和评价两者产仔率和3167个sows.ResultsThe结果产仔表明SP-OXT公猪（之间和公猪精液内不同而异P <0.05），但不品种（杜洛克，皮特兰，长白猪和大白猪）之间。精液具有较高SP-OXT / mL浓度（分层次进行分组; P <0.001）具有更大的体积和从年轻的公猪（P <0.05）来了。公猪表示阳性产仔率偏差的精液表现出较高（P <0.05）SP-OXT / mL浓度比那些OXT的负产仔率deviation.ConclusionThe SP浓度是野猪，精液和年龄依赖性，并与射精量和分娩正相关率的液体储存精液AI-剂量。
Padilla; Lorena[1];López-Arjona; Marina[1];Martinez-Subiela; Silvia[1];Rodriguez-Martinez; Heriberto[2];Roca; Jordi[1];Barranco; Isabel[3]. Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows[J]. Journal of Animal Science and Biotechnology, 2021,12(1): 1-11