期刊文献

Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region 收藏

CCHFV的核衣壳蛋白的抗原表位区域和鉴定的截短片段的序列表达
原文求助 发布源
作者
Peng-fei Wei [1] Yan-jun Luo [2] Tian-xian Li [2] Hua-lin Wang [2] Zhi-hong Hu [2] Fu-chun Zhang [1] Yu-jiang Zhang [3] Fei Deng [2] Su-rong Sun [1]
作者单位
1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830046, China 2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China 3. The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, 830002, China
关键词
Crimean-congo hemorrhagic fever virus (CCHFV) ;Expression ;Epitope ;Nucleocapsid protein (NP)
关键词译文
克里米亚 - 刚果出血热病毒（CCHFV）;​​表达;抗原表位;核蛋白（NP）
页码
45-51
DOI
10.1007/s12250-010-3067-7
来源信息
Virologica Sinica ISSN：1674-0769, 2010年, 25卷, 1期, 45-51页
摘要
The Crimean-congo hemorrhagic fever virus (CCHFV) is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP (nucleocapsid protein) gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum (rabbit) and 2 monoclonal (mAbs) (14B7 and 43E5) against CCHFV by Western-blot analyses. The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein.
摘要译文
克里米亚 - 刚果出血热病毒（CCHFV）是一个地域广泛的致命的病原体。病毒的表位区域的识别是用于诊断和CCHFV感染的流行病学研究很重要。在这项研究中，携带来自CCHFV应变YL04057在S片段的NP（核蛋白）基因的序列截短片段的表达载体。重组蛋白在大肠杆菌中表达并纯化，用于检测。用多克隆血清（兔）和2单克隆（单抗）（14B7和43E5）针对CCHFV通过Western印迹分析检测到的NP的截短片段的抗原。结果表明，三种表达构建体，其中包含的所有区域235AA到305AA可以通过单克隆抗体的多克隆血清来检测。结果表明，区域235-305氨基酸的NP是高度抗原区和高度保守的NP蛋白。
Peng-fei Wei [1] Yan-jun Luo [2] Tian-xian Li [2] Hua-lin Wang [2] Zhi-hong Hu [2] Fu-chun Zhang [1] Yu-jiang Zhang [3] Fei Deng [2] Su-rong Sun [1]. Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region[J]. Virologica Sinica, 2010,25(1): 45-51