摘要
We examined the effects of dietary supplementation of a multicomponent blend of prebiotics and probiotics on health, immune status, metabolism, and performance of newly weaned beef steers during a 35-d receiving period. Eighty newly weaned crossbred steers (12-hour postweaning; 206 ± 12 kg of body weight [BW]) from a single source were stratified by BW into four pens (20 steers per pen) such that each pen had similar BW at the beginning of the experiment. The pens were randomly assigned to receive a corn silage-based diet with no additive (CON; two pens; n = 40 steers) or a basal diet supplemented with SYNB feed additive at an average of 28 g/steer/d (SYNB; two pens; n = 40 steers). The SYNB additive is a blend of live Saccharomyces cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis and was supplemented for the first 21 d only. Percentage of steers treated for bovine respiratory disease (BRD) was calculated for each dietary treatment. Daily dry matter intake (DMI) and meal events (meal frequency and duration) were measured. Weekly BWs were measured to calculate average daily gain (ADG). Blood samples collected on days 0, 14, 21, 28, and 35 were used for ex-vivo tumor necrosis factor alpha (TNF-α) release assay following lipopolysaccharides (LPS) stimulation, plasma metabolome analysis, and mRNA expression analysis of 84 innate and adaptive immune-related genes. Compared with CON, supplemental SYNB increased (P ≤ 0.05) ADG, DMI, and meal events during the first 7 d. At d 21, there was no treatment effect (P > 0.05) on final BW, DMI, ADG, and meal events; however, beef steers fed supplemental SYNB had greater (P = 0.02) meal duration. Over the entire 35-d receiving period, beef steers fed supplemental SYNB had greater (P = 0.01) ADG and feed efficiency, tended to have greater (P = 0.08) meal duration, and had lower percentage (35 vs. 50%) of animals treated for BRD and lower percentage of sick animals treated for BRD more than once (7.15 vs. 45%). Whole blood expression of pro-inflammatory genes was downregulated while that of anti-inflammatory genes was upregulated in beef steers fed supplemental SYNB. Beef steers fed supplemental SYNB had lower (P = 0.03) plasma concentration of TNF-α after LPS stimulation. Six nutrient metabolic pathways associated with health benefits were enriched (false discovery rate ≤ 0.05) in beef steers fed supplemental SYNB. This study demonstrated that dietary supplementation of SYNB during the first 21 d of arrival reduced BRD morbidity, improved the performance, immune, and metabolic status of beef steers over a 35-d receiving period thereby extending the SYNB effect by a further 14 days post supplementation.
摘要译文
我们研究了饮食中补充益生元和益生菌的多组分混合物对健康，免疫状态，代谢以及在35D接受期内新断奶牛肉ste牛的影响的影响。从单个来源将八十种新奶的杂交stre（断奶后12小时； 206±12千克体重[BW]）通过BW分层为四支笔（每笔20个笔式）本实验。将笔随机分配以接受基于玉米青贮饲料的饮食，没有添加剂（con;两支笔； n = 40辆）或补充合成饲料添加剂的基础饮食，平均为28 g/steer/d（合成；两个）笔; n = 40转向）。合成添加剂是酿酒酵母的活糖疗和酿酒酵母，乳酸肠球菌，乳杆菌芽孢杆菌和枯草芽孢杆菌的发酵产物的混合物，仅在前21 d中得到补充。计算每种饮食疗法的牛呼吸道疾病（BRD）的蒸汽百分比。测量每日干物质摄入量（DMI）和进餐事件（进餐频率和持续时间）。测量每周的BW以计算平均每日增益（ADG）。在脂多糖刺激（LPS）刺激后，使用第0、14、21、28和35天收集的血液样本进行外肿瘤坏死因子α（TNF-α）释放测定法，血浆代谢组分析和84个先天性的MRNA表达分析和自适应免疫相关基因。与CON相比，在前7 d期间，补充合成增加（P≤0.05）ADG，DMI和进餐事件。在D 21时，最终BW，DMI，ADG和进餐事件没有治疗效果（p> 0.05）。但是，饲喂补充合成的牛肉蒸汽持续时间更大（p = 0.02）。在整个35D接收期内，饲喂补充合成的牛肉转向均具有更大的ADG（p = 0.01）和饲料效率，往往具有更大的（p = 0.08）的饮食时间，并且百分比较低（35 vs. 50％）接受过BRD治疗的动物和较低的BRD治疗的动物百分比多次（7.15 vs. 45％）。促炎基因的全血表达被下调，而抗炎基因的全血表达在喂养补充合成的牛肉转向中上调。 LPS刺激后，饲喂补充合成的牛肉均具有较低（p = 0.03）血浆TNF-α。与健康益处相关的六种营养代谢途径富集（错误发现率≤0.05）喂养补充合成。这项研究表明，在到达的前21天中补充饮食降低了BRD发病率，改善了35D接收期内牛肉ste牛的性能，免疫和代谢状态，从而在补充后再增加14天。
Modoluwamu D Idowu [1]; Godstime Taiwo [1]; Andres Pech Cervantes [2]; Scott A Bowdridge [1]; Ibukun M Ogunade [1]. Effects of a multicomponent microbial feed additive containing prebiotics and probiotics on health, immune status, metabolism, and performance of newly weaned beef steers during a 35-d receiving period[J]. Translational Animal Science, 2022,6(2)