摘要
Sex-control technology have great economic value and is one of the hot topics in livestock research. To produce more milk, dairy farmers prefer female offspring. X/Y sperm separation is an effective method for offspring sex control. Currently, the major commercial production method for sperm separation is flow cytometry sorting in cattle. However, flow cytometry requires expensive equipment and long sorting times. So, a simple and inexpensive method for producing a higher number of dairy cows is required. In this study, R848 activates toll-like receptor 7/8 (TLR7/8), thereby separating X from Y sperm. The results showed TLR7/8 is expressed in the tail of X sperm. Immunofluorescence (IF) of testes, epididymis, and ejaculate shows that the number of TLR7+/8+ sperm cells is up to 50 %. Furthermore, TLR7/8 agonist (R848) affects mitochondrial function through the PI3K/GSK3α/β/hexokinase and PI3K/NFκB/hexokinase signalling pathways, inhibiting X sperm motility, while the motility of Y-sperm remains unchanged. The difference in sperm motility causes Y sperm (with high motility) to move to the upper layer and X-sperm (with low motility) to the lower layer allowing the separation of X and Y sperm. Based on this study, we reveal a simple and effective method for enriched X/Y sperms from cattle.
摘要译文
性别控制技术具有巨大的经济价值，并且是牲畜研究中的热门话题之一。为了生产更多的牛奶，奶农更喜欢女性后代。X/Y精子分离是后代性别控制的有效方法。目前，精子分离的主要商业生产方法是牛流式细胞术分选。但是，流式细胞仪需要昂贵的设备和较长的分类时间。因此，需要一种简单且廉价的方法来产生更高数量的奶牛。在这项研究中，R848激活类似Toll样受体7/8（TLR7/8），从而将X与Y精子分开。结果表明TLR7/8在X精子的尾部表达。睾丸，附睾和射精的免疫荧光（IF）表明，TLR7 +/8 +精子细胞的数量高达50％。此外，TLR7/8激动剂（R848）通过PI3K/GSK3α/β/Hexokinase和PI3K/NFκB/Hexokinase信号传导途径影响线粒体功能，抑制X精子运动，而Y-Sperm的运动性保持不变。精子运动的差异导致Y精子（具有高运动性）移至上层，X-Sperm（低运动性）向下层移动，从而使X和Y精子分离。基于这项研究，我们揭示了一种简单有效的方法，可用于牛的富集X/Y精子。
Fei Wen; Weidong Liu; Yu Li; Qianlong Zou; ... Jianhong Hu. TLR7/8 agonist (R848) inhibit bovine X sperm motility via PI3K/GSK3α/β and PI3K/NFκB pathways[J]. International Journal of Biological Macromolecules, 2023,232