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Dietary dragonhead effects on growth, immunity and antioxidant and related genes expression in zebrafish (Danio rerio) 收藏

饮食龙头对斑马鱼（Danio Rerio）中生长，免疫和抗氧化剂和相关基因表达的影响（Danio Rerio）
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作者
Roghieh Safari; Zahra Roosta; Farzaneh Vakili; Emad Rahmani; ... Seyed Hossein Hoseinifar
作者单位
[a]Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranDepartment of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran[b]Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, IranFisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran[c]Department of Fisheries, Sari University of Agricultural Sciences and Natural Resources, IranDepartment of Fisheries, Sari University of Agricultural Sciences and Natural Resources, Iran[d]School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranSchool of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran[e]Hagerman Fish Culture Experiment Station, University of Idaho, 3059F National Fish Hatchery Road, Hagerman, ID 83332, USAHagerman Fish Culture Experiment Station, University of Idaho, 3059F National Fish Hatchery Road, Hagerman, ID 83332, USA[f]Food, Drug and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, IranFood, Drug and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran[g]Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand[h]Department of Science and Technologies, Via De Sanctis, University of Sannio, 82100 Benevento, ItalyDepartment of Science and Technologies, Via De Sanctis, University of Sannio, 82100 Benevento, Italy[i]Science and Technology Research Institute, Chiang Mai University, 239 Huay Keaw Rd., Suthep, Muang, Chiang Mai, 50200, ThailandScience and Technology Research Institute, Chiang Mai University, 239 Huay Keaw Rd., Suthep, Muang, Chiang Mai, 50200, Thailand
关键词
Dracocephalum kotschyi;Zebrafish;Immunity;Antioxidant defence;
关键词译文
Dracocephalum kotschyi;斑马鱼；免疫；抗氧化剂防御；
发布日期
1 December 2022
DOI
10.1016/j.aqrep.2022.101384
来源信息
Aquaculture Reports ISSN：2352-5134, 2022年, 27卷
摘要
The present study aimed to investigate the effects of the herbaceous plant, Dracocephalum kotschyi, on the immune response, antioxidant enzymes activity, growth performance, and related genes expression in zebrafish (Danio rerio). An in vivo trial was carried out by feeding zebrafish (initial weight of 0.47 ± 0.04 g) with different levels of powdered D. kotschyi: 0% (C-0%, control), 1% (Dk-1%), 2% (Dk-2%) and 3% (Dk-3%) for 10 weeks. The results show that total protein (TP) and total immunoglobulin (IG), were significantly increased in both skin mucus and whole body extract (WBE). Lysozyme (LYS) activity significantly increased in the WBE of zebrafish fed with D. kotschyi integrated diets compared to the control (P < 0.05). The superoxide dismutase (SOD) of WBE was significantly (P < 0.05) enhanced by dietary D. kotschyi. Additionally, this herb brought about a significant up-regulation in immune (LYS), pro-inflammatory (IL-1β) from intestine, and antioxidant (SOD and CAT) genes in liver, compared to the non-supplemented group (P < 0.05). Growth hormone (GH) and insulin growth factor (IGF) genes expression were upregulated significantly in zebrafish by the addition of D. kotschyi, although body weight did not change. The best results were obtained with the 3% inclusion of powdered D. kotschyi, except for LYS and IGF expressions that were best upregulated by 2% and 3% of D. kotschyi. Our results indicate that powdered D. kotschyi (suggested dose of 3%) could potentially improve zebrafish's immune response and antioxidant defense.
摘要译文
本研究旨在研究草本植物Dracocephalum kotschyi对免疫反应，抗氧化酶的活性，生长性能和相关基因表达的影响。通过喂食斑马鱼（初始重量为0.47±0.04 g），具有不同水平的粉末状的D. Kotschyi：0％（C-0％，对照），1％（DK-1％），进行了体内试验。（DK-2％）和3％（DK-3％）10周。结果表明，在皮肤粘液和全身提取物（WBE）中，总蛋白（TP）和总免疫球蛋白（IG）均显着增加。与对照组相比，用D. kotschyi综合饮食的斑马鱼的WBE溶菌酶（LYS）的活性显着增加（p <0.05）。WBE的超氧化物歧化酶（SOD）显着（p <0.05），饮食D. Kotschyi增强了。此外，与非抑制组相比）。尽管体重没有变化，但斑马鱼中的生长激素（GH）和胰岛素生长因子（IGF）基因表达在斑马鱼中显着上调。最好的结果是通过3％的粉末状d. kotschyi含量，除了Lys和IGF表达式最佳上调的Lys和IGF表达外，占2％和3％的D. kotschyi。我们的结果表明，粉末状的D. kotschyi（建议的剂量为3％）可能会改善斑马鱼的免疫反应和抗氧化剂防御。
Roghieh Safari; Zahra Roosta; Farzaneh Vakili; Emad Rahmani; ... Seyed Hossein Hoseinifar. Dietary dragonhead effects on growth, immunity and antioxidant and related genes expression in zebrafish (Danio rerio)[J]. Aquaculture Reports, 2022,27