摘要
Black gamju is Korean traditional beverage fermented with molds. The aim of this study was to assess the probiotic properties and antioxidant activities of novel Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 to develop black gamju with bioactive properties for health. Tolerance against artificial gastric juice and bile salts, adhesion ability on HT-29 cells of strains, and antibiotics susceptibility were evaluated as probiotics, and various enzyme productions were detected. The 2,2-diphenyl-1-picrylhydrazyl assay, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate, and β-carotene bleaching assay were used for antioxidant activity of samples. The tolerance of both strains to artificial gastric juice and bile salts (Oxgall) was more than 90%. Additionally, both strains did not produce β-glucuronidase and were resistant to gentamicin, kanamycin, streptomycin, and ciprofloxacin. After fermentation of black gamju with each strain, the number of viable lactic acid bacteria increased to 8.25–8.95 log colony forming unit/mL, but the pH value of fermented samples decreased more (to pH 3.33–3.41) than that of control (pH 4.37). L. brevis KU15151 showed higher adhesion activity to HT-29 cells and antioxidant effects than P. pentosaceus SC28 in three antioxidant assays.
摘要译文
黑色格兰朱是韩国传统饮料，用霉菌发酵。本研究的目的是评估新型Pediococcus Pentosaceus SC28和Levilactobacillus Brevis Ku15151的益生菌性质和抗氧化活性，以发育黑色Gamju，具有健康的生物活性性能。评价菌株HT-29细胞的耐受人造胃液和胆汁盐，粘附能力，以及抗生素易感性被评价为益生菌，并检测各种酶生产。 2,2-二苯基-1-富铬酰基测定，2,2'-α-吲哚（3-乙基异噻唑啉-6-磺酸盐和β-胡萝卜素漂白测定用于样品的抗氧化活性。菌株对人造胃液和胆汁盐（Oxgall）的耐受性大于90％。另外，两个菌株都没有产生β-葡糖醛酸酶，并且对庆大霉素，卡那霉素，链霉素和环丙沙星耐药。在用每种菌株发酵黑色格林厅后，可行的乳酸菌的数量增加到8.25-8.95个对数菌落形成单元/ ml，但发酵样品的pH值比对照（pH值）降低更多（pH3.33-3.41） 4.37）。 L. Brevis Ku15151对HT-29细胞和抗氧化效应显示出比P.Pentosaceus SC28在三种抗氧化剂测定中的抗氧化效应更高。
Seo Jin Yang[ 1]; Kee-Tae Kim[ 1]; Tae Young Kim[ 2];Hyun-Dong Paik[ 1;[*]. Probiotic Properties and Antioxidant Activities of Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 in Fermented Black Gamju[J]. Foods, 2020,9(9): 1154