摘要
This study aimed to characterize the rumen bacterial diversity of beef steers differing in the efficiency of nitrogen retention (ENR). Eight castrated steers and fitted with ruminal silicone - and duodenal T-type cannulas were used in a cross-over design with three consecutive periods and three diets. During each experimental period, nitrogen balance was measured, and based on the efficiency of N utilization data, steers were split into three ENR groups: high (HNR, 56.6% ± 3.3%, n = 10), medium (MNR, 45.8% ± 2.2%, n = 6), and low (LNR, 37.7% ± 1.9%, n = 8) using the NbClust package version 2.0.4 in R. Prevotellaceae, Lactobacillaceae, Leuconostocaceae, Clostridiales_Incertae_Sedis_XIII, Lachnospiraceae, and Peptostreptococcaceae were more abundant in LNR (P < 0.05) compared to HNR or MNR. Negative correlations were found between N retention and Mogibacterium, Anaerofustis, Butyrivibrio, Coprococcus, Hespellia, Lactonifactor and Lachnospiraceae (r ≤ −0.61; P ≤ 0.05). Prevotella, Hespellia, Lactonifactor, Lachnospiraceae_other, and Anaerobiospirillum were positively correlated between urinary N excretion (r > 0.55; P < 0.01), and negative correlations were found with Elusimicrobia, Victivallis and Treponema (r < −0.41; P < 0.05). The adjustment of the rumen bacterial community differed significantly between the N use retention groups. The high N retention in beef cattle was associated with less abundant bacteria in the rumen; however, N fixation capacity and uncharacterized rumen microorganisms need to be elucidated in future studies. In contrast, lower N utilization was associated with high abundance of bacteria that promote greater urinary N excretion through ruminal protein degradation.
摘要译文
本研究旨在表征牛肉带的瘤胃细菌多样性，其氮潴留效率（ENR）。八个阉割配套器和配有瘤型硅胶 - 和十二指肠T型套管用于交叉设计，连续三个时期和三个饮食。在每个实验期间，测量氮气平衡，并基于N利用数据的效率，将带状者分成三种巢组：高（HNR，56.6％±3.3％，N = 10），培养基（MNR，45.8％± 2.2％，n = 6），低（LNR，37.7％±1.9％，n = 8）使用Nbclust包3.0.4在R.Fvotellaceae，Lactobacilaceraceae，Leuconostocaceae，Clostridiales_Incertae_sedis_Xiii，Lachnospiraceae和Peptostrococaceae更丰富与HNR或MNR相比，LNR（P <0.05）。在N保留和Mogibacterium，Anaerofustis，抑虫剂，豆科病症，肝细胞，乳酸酐和Lachnospireae（R≤-0.61; p≤0.05）。 PREVOTLA，HESPLELIA，Lactonifactor，Lachnospiraceae_other和Anaerobiospirill在尿n排泄（R> 0.55之间是正相关的P <0.01），用ELUSIMICROBIA，VIRTIVALLIS和TREPONEMA（R <-0.41; P <0.05）。瘤胃细菌群落的调节在N使用保留组之间显着不同。牛肉中的高N保留与瘤胃中较少的细菌相关;然而，在未来的研究中需要阐明N个固定能力和无表特征瘤胃微生物。相比之下，较低的N利用率与高丰度的细菌有关，通过瘤胃蛋白质降解促进更大的尿液N排泄。
Kênia LarissaGomes Carvalho Alves[a];Yury TatianaGranja-Salcedo[a][e];Juliana DuarteMessana[a];ViníciusCarneiro de Souza[a][f];Maria JúliaGeneroso Ganga[a];Paulo HenriqueDetogni Colovate[a];Luciano TakeshiKishi[b][c];Telma TeresinhaBerchielli[a][d];. Rumen bacterial diversity in relation to nitrogen retention in beef cattle - ScienceDirect[J]. Anaerobe, 2021,67