摘要
Karenia mikimotoi is one of the most well-known harmful bloom species in temperate coastal waters. The present study investigated the characteristics of alkaline phosphatase (APase) and phosphodiesterase (PDEase) activities in hydrolysis of two phosphomonoesters (adenosine triphosphate (ATP) and ribulose 5-phosphate (R5P)) and a phosphodiester (cyclic adenosine monophosphate (cAMP)) in K. mikimotoi and compared its growth and physiological responses to the different forms of phosphorus substrates. K. mikimotoi produced comparable quantities of APase and PDEase to hydrolyze the organic phosphorus substrates, although hydrolysis of the phosphomonoesters was much faster than that of the phosphodiester. The growth of K. mikimotoi on organic phosphorus substrates was comparable to or better than that on inorganic phosphate. The difference in particulate organic nutrients (carbon, nitrogen, and phosphorus) and hemolytic activity supported different rates of hydrolysis-assimilation of the various organic phosphorus substrates by K. mikimotoi. The hemolytic activities of K. mikimotoi in the presence of organic phosphorus substrates were several times those in the presence of inorganic phosphate during the exponential phase. This suggested the potential important role of organic phosphorus in K. mikimotoi blooms.
摘要译文
Karenia Mikimotoi是温带温带沿海水域最着名的有害绽放物种之一。本研究研究了两种磷酸族聚酯水解中碱性磷酸酶（Apase）和磷酸二酯酶（Pdease）活性的特性（腺苷三磷酸（ATP）和核苷酸5-磷酸（R5P））和磷酸二磷酸酯（环腺苷一磷酸盐（营地）） K.Mikimotoi并将其生长和生理反应与不同形式的磷底物进行比较。 K.Mikimotoi生产了可比量的嗜睡和PDEASE以水解有机磷基材，尽管磷酸磷酸酯的水解比磷酸二酯的水解得多。 K.Mikimotoi在有机磷基材上的生长与无机磷酸盐的含量相当或更好。颗粒有机营养素（碳，氮气和磷）和溶血活性的差异支持通过K.Mikimotoi的各种有机磷基质的水解同化率的不同速率。 K.MikimotoI在有机磷基质存在下的溶血活性是在指数期期间无机磷酸盐存在的几倍。这表明有机磷在K.Mikimotoi盛开的潜在重要作用。
Kaixuan Huang;Yanqing Zhuang;Zhou Wang;Linjian Ou;Jingyi Cen;Songhui Lu;Yuzao Qi. Bioavailability of Organic Phosphorus Compounds to the Harmful Dinoflagellate Karenia mikimotoi[J]. Microorganisms, 2021,9(9): 1961