期刊文献

Pumpkin extract and fermented whey individually and in combination alleviated AFB1- and OTA-induced alterations on neuronal differentiation in vitro 收藏

南瓜提取物和发酵乳清单独和组合减轻了AFB1和OTA诱导的体外神经元分化的改变

原文求助发布源

作者

Massimo Frangiamone[1];Manuel Alonso-Garrido[1];Guillermina Font;Alessandra Cimbalo;Lara Manyes

作者单位

Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, Universitat de València, Vicent Andrés Estellés s/n, 46100, Burjassot, SpainLaboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, Universitat de Valencia, Vicent Andres Estelles s/n, 46100, Burjassot, Spain[1]1Both authors contributed equally.

关键词

Mycotoxins;Bioactive compounds;Neurotoxicity;Immunofluorescence;qPCR;

关键词译文

霉菌毒素；生物活性化合物；神经毒性；免疫荧光； QPCR；

发布日期

1 June 2022

DOI

10.1016/j.fct.2022.113011

来源信息

Food and Chemical Toxicology ISSN：0278-6915, 2022年, 164卷

摘要

Food and feed are daily exposed to mycotoxin contamination which effects may be counteracted by functional compounds like carotenoids and fermented whey. Among mycotoxins, the most toxic and studied are aflatoxin B1 (AFB1) and ochratoxin A (OTA), which neurotoxicity is not well reported. Therefore, SH-SY5Y human neuroblastoma cells ongoing differentiation were exposed during 7 days to digested bread extracts contained pumpkin and fermented whey, individually and in combination, along with AFB1 and OTA and their combination, in order to evaluate their presumed effects on neuronal differentiation. The immunofluorescence analysis of βIII-tubulin and dopamine markers pointed to OTA as the most damaging treatment for cell differentiation. Cell cycle analysis reported the highest significant differences for OTA-contained bread compared to the control in phase G₀/G₁. Lastly, RNA extraction was performed and gene expression was analyzed by qPCR. The selected genes were related to neuronal differentiation and cell cycle. The addition of functional ingredients in breads not only enhancing the expression of neuronal markers, but also induced an overall improvement of gene expression compromised by mycotoxins activity. These data confirm that in vitro neuronal differentiation may be impaired by AFB1 and OTA-exposure, which could be modulated by bioactive compounds naturally found in diet.

摘要译文

食物和饲料每天暴露于霉菌毒素污染中，这些污染物可能会被类胡萝卜素和发酵乳清等功能化合物所抵消。在霉菌毒素中，最毒性和研究的是黄曲霉毒素B1（AFB1）和ochratoxin A（OTA），这些神经毒性尚未得到很好的报道。因此，在7天内，SH-SY5Y人类神经母细胞瘤细胞正在暴露于消化的面包提取物中，含有南瓜和发酵乳清，单独和组合，以及AFB1和OTA及其组合，以评估其推测的对神经元分化的影响。 βIII-微管蛋白和多巴胺标志物的免疫荧光分析指出，OTA是细胞分化最具破坏性的治疗方法。与阶段G 0 /G 1中的对照相比，细胞周期分析报道了含有OTA的面包的显着差异最高。最后，进行RNA提取，并通过qPCR分析基因表达。选定的基因与神经元分化和细胞周期有关。面包中的功能成分不仅增强了神经元标记的表达，而且还引起了霉菌毒素活性损害的基因表达的总体改善。这些数据证实，AFB1和OTA暴露可能会损害体外神经元分化，这可以通过饮食中自然发现的生物活性化合物来调节。

Massimo Frangiamone[1];Manuel Alonso-Garrido[1];Guillermina Font;Alessandra Cimbalo;Lara Manyes. Pumpkin extract and fermented whey individually and in combination alleviated AFB1- and OTA-induced alterations on neuronal differentiation in vitro[J]. Food and Chemical Toxicology, 2022,164