摘要
Perilla frutescens (L.) Britt., an annual herbaceous plant, has antibacterial, anti-inflammation, and antioxidant properties. To understand the effects of P. frutescens leaf on the ruminal microbial ecology of cattle, Illumina MiSeq 16S rRNA sequencing technology was used. Fourteen cows were used in a randomized complete block design trial. Two diets were fed to these cattle: a control diet (CON); and CON supplemented with 300 g/d P. frutescens leaf (PFL) per cow. Ruminal fluid was sampled at the end of the experiment for microbial DNA extraction. Overall, our findings revealed that supplementation with PFL could increase ruminal fluid pH value. The ruminal bacterial community of cattle was dominated by Bacteroidetes, Firmicutes, and Proteobacteria. The addition of PFL had a positive effect on Firmicutes, Actinobacteria, and Spirochaetes, but had no effect on Bacteroidetes and Proteobacteria compared with the CON. The supplementation with PFL significantly increased the abundance of Marvinbryantia, Acetitomaculum, Ruminococcus gauvreauii, Eubacterium coprostanoligenes, Selenomonas_1, Pseudoscardovia, norank_f__Muribaculaceae, and Sharpea, and decreased the abundance of Treponema_2 compared to CON. Eubacterium coprostanoligenes, and norank_f__Muribaculaceae were positively correlated with ruminal pH value. It was found that norank_f__Muribaculaceae and Acetitomaculum were positively correlated with milk yield, indicating that these different genera are PFL associated bacteria. This study suggests that PFL supplementation could increase the ruminal pH value and induce shifts in the ruminal bacterial composition.
摘要译文
Perilla Frutescens（L.）Britt。，一年一度的草本植物，具有抗菌，抗炎和抗氧化性能。要了解P. Frutescens Leaf对牛瘤胃微生物生态的影响，使用Illumina Miseq 16S RRNA测序技术。在随机的完整块设计试验中使用了十四奶牛。将两种饮食送入这些牛：治疗饮食（CON）;并补充每头牛300克/ D P. Frutescens叶（PFL）。在微生物DNA提取的实验结束时对瘤胃流体进行采样。总体而言，我们的研究结果表明，用PFL补充可以增加瘤胃液pH值。牛的脊髓细菌群落由拟菌，压制和植物占主导地位。 PFL的添加对压力，肌肌细菌和螺旋体具有积极影响，但与孔相比，对菌体和噬菌体没有影响。对PFL的补充显着增加了Marvinbryantia，醋酸纤维素，谣言，牙龈葡萄球菌，番茄菌，索伦胺_1，伪坐和Sharkea，并降低了与孔相比的丰度。蚕豆豆荚和NoRank_f__muribaceae与瘤胃pH值正相关。结果发现，Norank_F_MURIBACALEAE和醋酸纤维素与牛奶产率正相关，表明这些不同的属是PFL相关细菌。该研究表明，PFL补充可以增加瘤胃pH值，并诱导瘤胃细菌组合物的换档。
Zhiqiang Sun; Zhu Yu;Bing Wang[ [*]. Perilla frutescens Leaf Alters the Rumen Microbial Community of Lactating Dairy Cows[J]. Microorganisms, 2019,7(11): 562