摘要
This study investigated the effects of replacing fish meal (FM) with fermented soybean meal (FSM) on the growth performance, serum biochemical indices, intestinal health, microbial community and disease resistance of largemouth bass (Micropterus salmoides). The basal diet was designed to contain 350 g/kg FM (control, FSM-0), and then, FM was decreased to 300 g/kg, 250 g/kg, 200 g/kg, 150 g/kg by the FSM inclusion of 75 g/kg (FSM-7.5), 150 g/kg (FSM-15.0), 225 g/kg (FSM-22.5) and 300 g/kg (FSM-30), respectively. The five diets were fed to largemouth bass with initial body weight of 21.2 ± 0.1 g for 8 weeks. The results showed that FSM-30 group presented lower weight gain, and FSM-22.5 and FSM-30 groups displayed higher feed conversion ratio than those of the control group (P < 0.05). No significant difference was observed in whole body composition (moisture, crude protein, crude lipid and crude ash) among all the groups (P > 0.05). Compared with the control, the protein retention in FSM-30 group, the lipid retention in FSM-15, FSM-22.5 and FSM-30 groups, and serum superoxide dismutase activity and total antioxidant capacity in FSM-22.5 and FSM-30 groups were decreased (P < 0.05). Moreover, the foregut villus height was reduced in all the FM-replaced groups, but the villus height, muscle thickness and villus circumferences of foregut were promoted in FSM-15, FSM-22.5 and FSM-30 groups. In terms of intestinal microorganism, FSM-22.5 and FSM-30 groups had lower abundance of Cetobacterium and higher abundance of Mycoplasma than those of the control group (P < 0.05). In the challenge test with intraperitoneal injection of Aeromonas hydrophila, the cumulative mortality was lowered in FSM-22.5 group (P < 0.05). In conclusion, in a basal diet containing 350 g/kg FM, FSM could be included at 150 g/kg to replace 100 g/kg FM without negative effects on the growth, feed utilization and intestinal health of largemouth bass.
摘要译文
本研究调查了用发酵大豆膳食（FSM）替代鱼粉（FM）对巨口鲈鱼（MicroPerulus Salmoides）的生长性能，血清生化指数，血清生化指数，微生物群落和抗病性的影响。基础饮食设计为含有350g / kg fm（对照，fsm-0），然后，通过FSM夹杂物将Fm降低至300g / kg，250g / kg，200g / kg，150g / kg 75g / kg（FSM-7.5），150g / kg（FSM-15.0），225g / kg（FSM-22.5）和300g / kg（FSM-30）分别。将五种饮食饲喂到大型鲈鱼，初始体重21.2±0.1g 8周。结果表明，FSM-30组呈较低的重量增益，FSM-22.5和FSM-30组的饲料转化率较高（P <0.05）。在所有组中，全身组成（水分，粗蛋白质，粗脂质和粗灰）中没有观察到显着差异（p> 0.05）。与对照相比，FSM-30组中的蛋白质保留，FSM-15，FSM-22.5和FSM-30组中的脂质保留，以及FSM-22.5和FSM-30组中的血清超氧化物歧化酶活性和总抗氧化能力减少（P <0.05）。此外，在所有FM替代的组中，预留绒毛高度降低，但绒毛高度，肌肉厚度和绒毛的周长在FSF-15，FSM-22.5和FSM-30组中促进。就肠道微生物而言，FSM-22.5和FSM-30组具有较低的唾液酸杆菌和高度高度的支原体比对照组（P <0.05）。在腹腔内注射航空宿龙患者患有肝细胞液的挑战试验中，FSM-22.5组中累积死亡率降低了（P <0.05）。总之，在含有350g / kg Fm的基础饮食中，FSM可以包括在150g / kg中，以替代100克/千克FM，对Largemouth Bass的生长，饲料利用和肠健康产生负面影响。
Hang Yang[a][d][e][1];Yuhao Bian[a][1];Lingling Huang[b][c];Qing Lan[b][c];Lizhou Ma[b][c];Xiaoqin Li[a][d][e];Xiangjun Leng[a][d][e]. Effects of replacing fish meal with fermented soybean meal on the growth performance, intestinal microbiota, morphology and disease resistance of largemouth bass (Micropterus salmoides)[J]. Aquaculture Reports, 2022,22