摘要
This study demonstrated the potential effects of the rumen microbiota on the deposition of intramuscular fat, known as marbling. Previous studies on fatty acid metabolism in beef cattle have mostly focused on biohydrogenating rumen bacteria, whereas those on the overall rumen microbiota—to understand their roles in marbling—have not been systematically performed. The rumen microbiota of 14 Korean beef cattle (Hanwoo), which showed similar carcass characteristics and blood metabolites but different marbling scores, were analyzed by 16S rRNA gene sequencing. The rumen samples were grouped into two extreme marbling score groups of host animals as follows: LMS, marbling score≤ 4 or HMS, marbling score ≥7. Species richness tended to be higher in the HMS group, whereas the overall microbiota differed between LMS and HMS groups. RFP12, Verrucomicrobia, Oscillospira, Porphyromonadaceae, and Paludibacter were differentially abundant in the HMS group, whereas Olsenella was abundant in the LMS group. Some marbling-associated bacterial taxa also contributed to the enrichment of two lipid metabolic pathways including “alpha-linolenic acid metabolism” and “fatty acid biosynthesis” in the HMS microbiome. Taxonomic drivers of fatty acid biosynthesis, particularly in the rumen microbiome of high-marbled meat, could thus be further studied to increase the intramuscular fat content.
摘要译文
本研究表明了瘤胃微生物瘤对肌内脂肪沉积的潜在影响，称为大理石。以前关于肉牛中脂肪酸代谢的研究主要集中在生物中的瘤胃细菌上，而那些关于整体瘤胃微生物群 - 以了解他们在大理石上的角色尚未得到系统性地进行。通过16S rRNA基因测序分析了14个韩国牛肉（Hanwoo）的瘤胃微生物，其显示出类似的胴体特征和血液代谢物，但血珠特征不同。将瘤胃样品分为两个极端的大理石筹分的宿主动物组：LMS，大理石比分≤4或HMS，大理石大理石比得分≥7。物种富含HMS组倾向于更高，而整体微生物会在LMS和HMS组之间不同。 RFP12，VerrucoMiCrobia，散蝇，卟啉单胞菌和Porudibacter在HMS组中差异丰富，而Olsenella在LMS组中很丰富。一些大理石的细菌分类群也有助于富集的两种脂质代谢途径，包括“α-亚麻酸代谢”和HMS微生物组中的“脂肪酸生物合成”。因此，可以进一步研究脂肪酸生物合成的分类脂肪酸的脂肪酸生物合成，特别是在高大理肉的瘤胃微生物中，以增加肌内脂肪含量。
Minseok Kim[ 1;2;†]; Tansol Park[ 3;†]; Jin Young Jeong[ 1]; Youlchang Baek[ 1];Hyun-Jeong Lee[ 4;[*]. Association between Rumen Microbiota and Marbling Score in Korean Native Beef Cattle[J]. Animals, 2020,10(4): 712